TY - JOUR
T1 - Zinc supplementation in normal conditions increases the pro-inflammatory cytokines
AU - Utomo, Martono T.
AU - Sudarmo, Subijanto M.
AU - Sudiana, Ketut
N1 - Publisher Copyright:
© 2019 JPR Solutions. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Background: Zinc supplementation in young adults has been found to increase dose-dependent pro-inflammatory cytokines. Increased proinflammatory cytokine has been found in severe sepsis and shock conditions. To determine the effect of zinc administration on normal conditions on pro-inflammatory cytokines compared to sepsis and normal conditions. Methods: A total sample of 40 rats was randomized into four control groups, lipopolysaccharide (LPS), LPS-zinc, and zinc. Placebo normal saline was given intravenously to the control and zinc groups, whereas in the LPS and LPS-zinc groups, intravenous Escherichia coli LPS was given. Blood collection was carried out at the 2nd h after administration to measure zinc levels, and an oral distilled water placebo was given to the control group and LPS, while the LPS-zinc and zinc groups were given zinc supplementation orally for 3 days. At 8, 24, and 72 h, blood was collected to measure tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) levels. At the 72nd h, blood was also taken to measure zinc levels. Examination of TNF-α and IL-6 levels used the enzymelinked immunosorbent assay Sandwich technique, while zinc levels used atomic absorption spectroscopy. Results: The TNF-α level in the zinc group at 72nd h was higher than the control group, while the zinc group’s IL-6 level was higher than the control group at 8th and 24th h. The levels of TNF-α and IL-6 in the zinc group were lower than LPS and LPS-zinc groups. Conclusions: Zinc supplementation under normal conditions increases the cytokines of TNF-α and IL-6.
AB - Background: Zinc supplementation in young adults has been found to increase dose-dependent pro-inflammatory cytokines. Increased proinflammatory cytokine has been found in severe sepsis and shock conditions. To determine the effect of zinc administration on normal conditions on pro-inflammatory cytokines compared to sepsis and normal conditions. Methods: A total sample of 40 rats was randomized into four control groups, lipopolysaccharide (LPS), LPS-zinc, and zinc. Placebo normal saline was given intravenously to the control and zinc groups, whereas in the LPS and LPS-zinc groups, intravenous Escherichia coli LPS was given. Blood collection was carried out at the 2nd h after administration to measure zinc levels, and an oral distilled water placebo was given to the control group and LPS, while the LPS-zinc and zinc groups were given zinc supplementation orally for 3 days. At 8, 24, and 72 h, blood was collected to measure tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) levels. At the 72nd h, blood was also taken to measure zinc levels. Examination of TNF-α and IL-6 levels used the enzymelinked immunosorbent assay Sandwich technique, while zinc levels used atomic absorption spectroscopy. Results: The TNF-α level in the zinc group at 72nd h was higher than the control group, while the zinc group’s IL-6 level was higher than the control group at 8th and 24th h. The levels of TNF-α and IL-6 in the zinc group were lower than LPS and LPS-zinc groups. Conclusions: Zinc supplementation under normal conditions increases the cytokines of TNF-α and IL-6.
KW - Interleukin-6
KW - Normal conditions
KW - Tumor necrosis factor-alpha
KW - Zinc
UR - http://www.scopus.com/inward/record.url?scp=85071503631&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85071503631
SN - 0975-7619
VL - 11
SP - 1983
EP - 1988
JO - Drug Invention Today
JF - Drug Invention Today
IS - 8
ER -