TY - JOUR
T1 - Zinc administration affects bronchial mucosal NF-κB p105/p50, p-NF-κB p65, IL-8, and IL-1β of zinc-deficient rats
AU - Ranuh, Reza Gunadi
AU - Darma, Andy
AU - Athiyyah, Alpha Fardah
AU - Latifolia, Ergia
AU - Setyoningrum, Retno Asih
AU - Hidajat, Boerhan
AU - Hidayati, Siti Nurul
AU - Endaryanto, Anang
AU - Sandra, Ferry
AU - Sudarmo, Subijanto Marto
N1 - Publisher Copyright:
© 2020 Prodia Education and Research Institute. All rights reserved.
PY - 2020/9
Y1 - 2020/9
N2 - BACKGROUND: Risk of acute respiratory infections up to 95%. in Zinc children deficiency less than is one 5 of years themain of age risk is factors. This study aimed to explore the effect of zinc on the bronchial mucosae inflammatory status expressed by nuclear factor (NF)-κB p105/p50, NF-κB p65, interleukin (IL)-8, and IL-1β. METHODS: Twenty-four Wistar rats were divided into 4 groups: normal zinc diet group without zinc supplementation (Z1), normal zinc diet group with zinc supplementation (Z2), zinc deficient diet group without zinc supplementation (Z3), and zinc deficient diet group with zinc supplementation (Z4). NF-κB p105/p50, p-NF-κB p65, IL-8, and IL-1β were measured by immunohistochemical staining. RESULTS: The inflammatory status of bronchial mucosae between Z1 and Z2 groups showed no difference (p=0.055). However, the inflammatory status of bronchial mucosae between Z3 and Z4 groups showed significant difference (p<0.01). Multivariate factorial design showed that zinc supplementation was beneficial when given to zinc deficient diet group with regard to decrease p-NF-κB p65, IL-8 and IL-1β levels (p<0.001) and increase dendritic cell (p=0.022). CONCLUSION: Zinc administration under conditions of zinc deficiency affects the inflammatory status, as shown by the decrease of p-NF-κB p65, IL-8 and IL-1β and the increase of NF-κB p105/p50.
AB - BACKGROUND: Risk of acute respiratory infections up to 95%. in Zinc children deficiency less than is one 5 of years themain of age risk is factors. This study aimed to explore the effect of zinc on the bronchial mucosae inflammatory status expressed by nuclear factor (NF)-κB p105/p50, NF-κB p65, interleukin (IL)-8, and IL-1β. METHODS: Twenty-four Wistar rats were divided into 4 groups: normal zinc diet group without zinc supplementation (Z1), normal zinc diet group with zinc supplementation (Z2), zinc deficient diet group without zinc supplementation (Z3), and zinc deficient diet group with zinc supplementation (Z4). NF-κB p105/p50, p-NF-κB p65, IL-8, and IL-1β were measured by immunohistochemical staining. RESULTS: The inflammatory status of bronchial mucosae between Z1 and Z2 groups showed no difference (p=0.055). However, the inflammatory status of bronchial mucosae between Z3 and Z4 groups showed significant difference (p<0.01). Multivariate factorial design showed that zinc supplementation was beneficial when given to zinc deficient diet group with regard to decrease p-NF-κB p65, IL-8 and IL-1β levels (p<0.001) and increase dendritic cell (p=0.022). CONCLUSION: Zinc administration under conditions of zinc deficiency affects the inflammatory status, as shown by the decrease of p-NF-κB p65, IL-8 and IL-1β and the increase of NF-κB p105/p50.
UR - http://www.scopus.com/inward/record.url?scp=85096898806&partnerID=8YFLogxK
U2 - 10.18585/inabj.v12i3.1041
DO - 10.18585/inabj.v12i3.1041
M3 - Article
AN - SCOPUS:85096898806
SN - 2085-3297
VL - 12
SP - 245
EP - 250
JO - Indonesian Biomedical Journal
JF - Indonesian Biomedical Journal
IS - 3
ER -