Water soluble protein optimized extraction from microalgae Spirulina platensis

This research aimed to determine the factors influencing extraction of water soluble protein from Spirulina platensis which included the ratio of Spirulina platensis and aquadest, incubation time, and interaction of those two factors. This research contained two factors (ratio and incubation time) using factorial complete randomized experimental design with nine treatments and three replications. Ratios of Spirulina platensis and aquadest used were 1:40, 1:50, and 1:60, while the incubation times were 1, 2 and 3 hours. Parameter observed in this study was water soluble protein content of Spirulina platensis. Ratio of Spirulina platensis and aquadest significantly affected water soluble protein content (p<0.05). The highest content of water soluble protein was observed at the ratio of 1: 50 (85.4742 μg/ml), while the highest water soluble protein content based on the incubation time was observed at 3 hours treatment (p<0.05). These results indicated that ratio and the incubation time influenced water soluble protein content on the extraction process of Spirulina platensis with the optimized extraction should be at the ratio of 1:50 for 3 hours.