TY - JOUR
T1 - Viral subtyping of HIV-1 derived from infected, drug-naive individuals in Jakarta, Indonesia
AU - Witaningrum, A. M.
AU - Khairunisa, S. Q.
AU - Ueda, S.
AU - Yunifiar, M. Q.
AU - Indriati, D. W.
AU - Kotaki, T.
AU - Rusli, A.
AU - Nasronudin, N.
AU - Kameoka, M.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2018/12/5
Y1 - 2018/12/5
N2 - Human immunodeficiency virus (HIV) infection and acquired immune deficiency syndrome (AIDS) (HIV/AIDS) remain a serious health problem in Indonesia. Jakarta has the largest number of individuals infected with HIV type 1 (HIV-1) in Indonesia; however, data on viral subtyping of HIV-1 is limited. We conducted a molecular epidemiological study on HIV-1 genes derived from infected, drug-naive individuals residing in Jakarta, the capital of Indonesia with large communities of HIV-infected individuals. To determine the distribution of HIV-1 subtypes circulating in Jakarta, 43 HIV-1-infected individuals were recruited in Jakarta, Indonesia. HIV-1 gag and envgenes were amplified from peripheral blood samples derived from study participants. Viral subtyping using the Recombinant Identification Program (RIP) and phylogenetic tree analysis were conducted. RIP and phylogenetic tree analysis revealed that CRF01-AE was the dominant subtype in Jakarta (40%), followed by recombinant viruses containing CRF01-AE and subtype B gene fragments (7%) and subtype B (7%). This study reported the emergence of CRF 01-AE as predominant subtype among HIV-1-infected, drug-naïve individuals in Jakarta, Indonesia. Therefore, continuous surveillance is required in order to detect the emergence of HIV-1-infection.
AB - Human immunodeficiency virus (HIV) infection and acquired immune deficiency syndrome (AIDS) (HIV/AIDS) remain a serious health problem in Indonesia. Jakarta has the largest number of individuals infected with HIV type 1 (HIV-1) in Indonesia; however, data on viral subtyping of HIV-1 is limited. We conducted a molecular epidemiological study on HIV-1 genes derived from infected, drug-naive individuals residing in Jakarta, the capital of Indonesia with large communities of HIV-infected individuals. To determine the distribution of HIV-1 subtypes circulating in Jakarta, 43 HIV-1-infected individuals were recruited in Jakarta, Indonesia. HIV-1 gag and envgenes were amplified from peripheral blood samples derived from study participants. Viral subtyping using the Recombinant Identification Program (RIP) and phylogenetic tree analysis were conducted. RIP and phylogenetic tree analysis revealed that CRF01-AE was the dominant subtype in Jakarta (40%), followed by recombinant viruses containing CRF01-AE and subtype B gene fragments (7%) and subtype B (7%). This study reported the emergence of CRF 01-AE as predominant subtype among HIV-1-infected, drug-naïve individuals in Jakarta, Indonesia. Therefore, continuous surveillance is required in order to detect the emergence of HIV-1-infection.
UR - http://www.scopus.com/inward/record.url?scp=85058327713&partnerID=8YFLogxK
U2 - 10.1088/1757-899X/434/1/012321
DO - 10.1088/1757-899X/434/1/012321
M3 - Conference article
AN - SCOPUS:85058327713
SN - 1757-8981
VL - 434
JO - IOP Conference Series: Materials Science and Engineering
JF - IOP Conference Series: Materials Science and Engineering
IS - 1
M1 - 012321
T2 - 3rd Annual Applied Science and Engineering Conference, AASEC 2018
Y2 - 18 April 2018
ER -
