Viability testof lemongrass extract (Cymbopogon citratus) on bhk-21 fibroblast cell culture

Shafira Kurnia Supandi, Yoshua Christian Michael, Lambang Bargowo

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Background: Lemongrass are found in Indonesia thus it lemongrass can be used as an alternative in healing inflammation by conducting a preliminary study in the form of BHK-21 fibroblast cell viability test after 24 hours of extract lemongrass. Objective: To determine the viability of extract lemongrass against BHK-21 fibroblast cell cultures. Method: BHK-21 fibroblast cells were taken from neonatal hamsters’ kidneys and planted in 96 well plates. Extracts lemongrass with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.13% and 1.56% were included in each well prepared and incubated for 24 hours. MTT Assay was performed to see the viability of extract lemongrass on fibroblast cells. Results: Extract lemongrass viable against BHK-21 fibroblast cells at a concentration of 100%. There is a decrease in viability at concentrations of 50% to 1.56%. Conclusion: The 100% concentration showed the highest viability of BHK-21 fibroblast cells and extract lemongrass had potential in wound healing.

Original languageEnglish
Pages (from-to)820-824
Number of pages5
JournalMedico-Legal Update
Issue number2
Publication statusPublished - 1 Apr 2020


  • BHK-21 fibroblast cells
  • Extract lemongrass
  • Viability


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