TY - JOUR
T1 - Viability Test of Hydroxyapatite Tooth Graft on Osteoblast cell culture
AU - Wijaya, Michael Ganda
AU - Prahasanti, Chiquita
AU - Laksono, Bambang Dwi
AU - Agrawanty, Westy
AU - Kusumawardhani, Banun
AU - Anggakusuma, Maria Jessica
N1 - Publisher Copyright:
© RJPT All right reserved.
PY - 2024/2
Y1 - 2024/2
N2 - Surgery is required to restore bone loss brought on by regenerative periodontal diseases while retaining the patient's aesthetics. The bone deficits caused by periodontal disease have been repaired using a variety of transplant materials. One of the graft materials used is dentin since it resembles bone in terms of both organic and inorganic components. In order to evaluate the viability of dental grafts, this study intended to count the osteoblast cells that were still alive after a specific therapy. Osteoblast cell cultures in 42 well plates were employed in this work. The 42 well plate cell cultures were separated into seven groups for 24hour examinations and seven groups for 48 hour examinations in order to examine the cells using the MTT assay. Each group contained control cells, control media devoid of cells, and the treatment group, which received tooth transplant at doses of 8, 4, 2, 1, and 0.5mg/mL. Using an ELISA reader with a 595nm wavelength, the optical density of these cells was used to determine the viability of the cells. There are more than 50% of osteoblast cells in all concentrations, which is indicated by the number of these cells. The Shapiro-Wilk, Levene, and Oneway Anova tests were performed to assess the normality, uniformity, and degree of group differences in the data. This study demonstrates the biocompatibility of the tooth graft and the osteoblast cells.
AB - Surgery is required to restore bone loss brought on by regenerative periodontal diseases while retaining the patient's aesthetics. The bone deficits caused by periodontal disease have been repaired using a variety of transplant materials. One of the graft materials used is dentin since it resembles bone in terms of both organic and inorganic components. In order to evaluate the viability of dental grafts, this study intended to count the osteoblast cells that were still alive after a specific therapy. Osteoblast cell cultures in 42 well plates were employed in this work. The 42 well plate cell cultures were separated into seven groups for 24hour examinations and seven groups for 48 hour examinations in order to examine the cells using the MTT assay. Each group contained control cells, control media devoid of cells, and the treatment group, which received tooth transplant at doses of 8, 4, 2, 1, and 0.5mg/mL. Using an ELISA reader with a 595nm wavelength, the optical density of these cells was used to determine the viability of the cells. There are more than 50% of osteoblast cells in all concentrations, which is indicated by the number of these cells. The Shapiro-Wilk, Levene, and Oneway Anova tests were performed to assess the normality, uniformity, and degree of group differences in the data. This study demonstrates the biocompatibility of the tooth graft and the osteoblast cells.
KW - Hydroxyapatite
KW - Osteoblast cells
KW - Tooth graft
KW - Viability
UR - http://www.scopus.com/inward/record.url?scp=85189978773&partnerID=8YFLogxK
U2 - 10.52711/0974-360X.2024.00132
DO - 10.52711/0974-360X.2024.00132
M3 - Article
AN - SCOPUS:85189978773
SN - 0974-3618
VL - 17
SP - 855
EP - 859
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 2
ER -