Viability of rabbit adipocyte stem cells cultured under different oxygen concentrations in vitro

Erma Safitri; Pudji Srianto; Thomas Valentinus Widiyatno; Willy Sandhika; R. Heru Prasetyo

Viability of rabbit adipocyte stem cells cultured under different oxygen concentrations in vitro

Erma Safitri, Pudji Srianto, Thomas Valentinus Widiyatno, Willy Sandhika, R. Heru Prasetyo

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The aim of this research was to determine if rabbit adipocyte stem cells (r-ASCs) can still be viable before transplantation when these are cultured in vitro under hypoxic preconditioning (3% O₂ concentration). Samples were divided into two groups, one under hypoxic preconditioning and the other in hyperoxia (20% O₂ concentration). Observations were made through flow cytometry, immunofluorescence and immunocytochemical analyses. Flow cytometric analysis showed that in r-ASCs hypoxic culture, the levels of CD90+, CD44+ and CD45- were unaltered, but changed under hyperoxic culture, indicated by the down-regulation of CD90+ and CD44+, and up-regulation of CD45-. Meanwhile, immunocytochemical and immunofluorescence analysis showed that under hypoxic preconditioning, r-ASCs culture expressed quiescent cells with p63 as marker, but the latter remained unexpressed in hyperoxic culture. In conclusion, hypoxic preconditioning with 3% O₂ concentration supported r-ASCs in sustaining viability before transplantation in rabbit.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Philippine Journal of Veterinary Medicine
Volume	55
Issue number	Special Issue
Publication status	Published - 1 Dec 2018

Keywords

Hyperoxia
Hypoxic preconditioning
R-ASCs
Rabbit
Viability

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title = "Viability of rabbit adipocyte stem cells cultured under different oxygen concentrations in vitro",

abstract = "The aim of this research was to determine if rabbit adipocyte stem cells (r-ASCs) can still be viable before transplantation when these are cultured in vitro under hypoxic preconditioning (3% O2 concentration). Samples were divided into two groups, one under hypoxic preconditioning and the other in hyperoxia (20% O2 concentration). Observations were made through flow cytometry, immunofluorescence and immunocytochemical analyses. Flow cytometric analysis showed that in r-ASCs hypoxic culture, the levels of CD90+, CD44+ and CD45- were unaltered, but changed under hyperoxic culture, indicated by the down-regulation of CD90+ and CD44+, and up-regulation of CD45-. Meanwhile, immunocytochemical and immunofluorescence analysis showed that under hypoxic preconditioning, r-ASCs culture expressed quiescent cells with p63 as marker, but the latter remained unexpressed in hyperoxic culture. In conclusion, hypoxic preconditioning with 3% O2 concentration supported r-ASCs in sustaining viability before transplantation in rabbit.",

keywords = "Hyperoxia, Hypoxic preconditioning, R-ASCs, Rabbit, Viability",

author = "Erma Safitri and Pudji Srianto and Widiyatno, {Thomas Valentinus} and Willy Sandhika and Prasetyo, {R. Heru}",

note = "Funding Information: This research was partially supported by the funding from the Directorate General of Higher Education (DIKTI) 2017, Ministry of National Education, Indonesia. Publisher Copyright: {\textcopyright} University of the Philippines at Los Banos. All rights reserved.",

year = "2018",

month = dec,

day = "1",

language = "English",

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T1 - Viability of rabbit adipocyte stem cells cultured under different oxygen concentrations in vitro

AU - Safitri, Erma

AU - Srianto, Pudji

AU - Widiyatno, Thomas Valentinus

AU - Sandhika, Willy

AU - Prasetyo, R. Heru

N1 - Funding Information: This research was partially supported by the funding from the Directorate General of Higher Education (DIKTI) 2017, Ministry of National Education, Indonesia. Publisher Copyright: © University of the Philippines at Los Banos. All rights reserved.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - The aim of this research was to determine if rabbit adipocyte stem cells (r-ASCs) can still be viable before transplantation when these are cultured in vitro under hypoxic preconditioning (3% O2 concentration). Samples were divided into two groups, one under hypoxic preconditioning and the other in hyperoxia (20% O2 concentration). Observations were made through flow cytometry, immunofluorescence and immunocytochemical analyses. Flow cytometric analysis showed that in r-ASCs hypoxic culture, the levels of CD90+, CD44+ and CD45- were unaltered, but changed under hyperoxic culture, indicated by the down-regulation of CD90+ and CD44+, and up-regulation of CD45-. Meanwhile, immunocytochemical and immunofluorescence analysis showed that under hypoxic preconditioning, r-ASCs culture expressed quiescent cells with p63 as marker, but the latter remained unexpressed in hyperoxic culture. In conclusion, hypoxic preconditioning with 3% O2 concentration supported r-ASCs in sustaining viability before transplantation in rabbit.

AB - The aim of this research was to determine if rabbit adipocyte stem cells (r-ASCs) can still be viable before transplantation when these are cultured in vitro under hypoxic preconditioning (3% O2 concentration). Samples were divided into two groups, one under hypoxic preconditioning and the other in hyperoxia (20% O2 concentration). Observations were made through flow cytometry, immunofluorescence and immunocytochemical analyses. Flow cytometric analysis showed that in r-ASCs hypoxic culture, the levels of CD90+, CD44+ and CD45- were unaltered, but changed under hyperoxic culture, indicated by the down-regulation of CD90+ and CD44+, and up-regulation of CD45-. Meanwhile, immunocytochemical and immunofluorescence analysis showed that under hypoxic preconditioning, r-ASCs culture expressed quiescent cells with p63 as marker, but the latter remained unexpressed in hyperoxic culture. In conclusion, hypoxic preconditioning with 3% O2 concentration supported r-ASCs in sustaining viability before transplantation in rabbit.

KW - Hyperoxia

KW - Hypoxic preconditioning

KW - R-ASCs

KW - Rabbit

KW - Viability

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SN - 0031-7705

VL - 55

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JO - Philippine Journal of Veterinary Medicine

JF - Philippine Journal of Veterinary Medicine

IS - Special Issue

ER -

Viability of rabbit adipocyte stem cells cultured under different oxygen concentrations in vitro

Abstract

Keywords

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