Viability of rabbit adipocyte stem cells cultured under different oxygen concentrations in vitro

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The aim of this research was to determine if rabbit adipocyte stem cells (r-ASCs) can still be viable before transplantation when these are cultured in vitro under hypoxic preconditioning (3% O2 concentration). Samples were divided into two groups, one under hypoxic preconditioning and the other in hyperoxia (20% O2 concentration). Observations were made through flow cytometry, immunofluorescence and immunocytochemical analyses. Flow cytometric analysis showed that in r-ASCs hypoxic culture, the levels of CD90+, CD44+ and CD45- were unaltered, but changed under hyperoxic culture, indicated by the down-regulation of CD90+ and CD44+, and up-regulation of CD45-. Meanwhile, immunocytochemical and immunofluorescence analysis showed that under hypoxic preconditioning, r-ASCs culture expressed quiescent cells with p63 as marker, but the latter remained unexpressed in hyperoxic culture. In conclusion, hypoxic preconditioning with 3% O2 concentration supported r-ASCs in sustaining viability before transplantation in rabbit.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalPhilippine Journal of Veterinary Medicine
Issue numberSpecial Issue
Publication statusPublished - 1 Dec 2018


  • Hyperoxia
  • Hypoxic preconditioning
  • R-ASCs
  • Rabbit
  • Viability


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