Nigella sativa toothpaste with antibacterial, anti-oxidant, and anti-inflammatory properties has beneficial effects in infectious disease, such as gingival inflammation. One of the most widely used synthetic detergents in toothpaste is Sodium lauryl sulphate (SLS). SLS is used to decrease water’s surface tension. The side effects of SLS include oral epithelial sloughing, ulcerations, inflammation, protein denaturation, and membrane expansion. This study aimed to determine the viability of human gingival fibroblast (HGFs) cultured with Nigella sativa toothpaste extract containing 2% SLS and Nigella sativa toothpaste without SLS. HGFs were grown in DMEM medium then challenged with Nigella sativa toothpaste with 2% SLS (Nigella sativa-SLS group), Nigella sativa toothpaste without SLS (non-SLS group), and control group. Based on the present study, the cell viability of Nigella sativa-2% SLS, non-SLS, and control group were 92.33%, 96.30%, and 98.99%, respectively. in conclusion, both Nigella sativa with 2% SLS and non-SLS were non-toxic to HGFs. Thus, Nigella sativa toothpaste is potential to treat gingivitis and oral diseases. However, future in vivo study for this is needed.

Original languageEnglish
Pages (from-to)525-528
Number of pages4
JournalJournal of International Dental and Medical Research
Issue number2
Publication statusPublished - 2021


  • Nigella sativa
  • human gingival fibroblasts
  • infectious disease
  • sodium lauryl sulphate
  • toothpaste


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