Vascular endothelial growth factor expression after induced by chicken shank collagen scaffold in bone regeneration

Vascular endothelial growth factor (VEGF) is an essential mediator during the process of angiogenesis that is an initial process of bone regeneration. Engineered bone tissue commonly encompass the use of scaffold. Collagen plays an important role in bone regeneration. Unfortunately, collagen can be extracted from chicken shank. This research was aimed to determine the effect of chicken shank collagen scaffold on the expresion of VEGF. Chicken shank was mixed by trypsin powder and dissolved in CH3COOH until forming like fiber then was sentifuged. Supernatant was dissolved NaCl 5wt% until forming fiber then was dialyzed for three days. Chicken shank collagen was printed in mould to freeze dry for 24 hours. Rats with defected femur were divided into 2 groups. Group 1 was the control group, and group 2 was treatment group which the defected femur were administrated with chicken shank collagen scaffold. Those rats were sacrificed on 2nd week. Tissue preparation was made then immunohistochemical staining was conducted counting VEGF expression. A statistical analysis was conducted using Mann Whitney test. There was a significant difference in the expression of VEGF (p < 0,05). The amount of VEGF expression was increased by chicken shank collagen scaffold.