TY - JOUR
T1 - Validity of Chemiluminescent Immunoassay Serology Test for Anti-Sars Cov-2 Antibodies IgM and IgG
AU - Museyaroh,
AU - Wardhani, Puspa
AU - Aryati,
N1 - Publisher Copyright:
© 2021, Indonesian Journal of Clinical Pathology and Medical Laboratory. All rights reserved.
PY - 2021/12/15
Y1 - 2021/12/15
N2 - In December 2019, an outbreak of acute pneumonia occurred in Wuhan, China. The disease was transmitted between humans through droplets (coughing or sneezing) of infected patients, causing this outbreak to spread rapidly in various countries in the world, including Indonesia. On February 11, 2020, WHO announced the pneumonia was caused by Coronavirus Disease 2019 (COVID-19), which was caused by a new type of Coronavirus, the SARS-CoV-2. A rapid and accurate diagnosis is critical for the control of the COVID-19 outbreak. The widely used test is a serology-based test that detects the presence of SARS-CoV-2 IgM/lgG antibodies in the patient's body. One of the methods used for this test is Chemiluminescent Immunoassay (CUA). This study aimed to determine the reliability of CUA. The study was conducted from August to September 2020. The number of samples was 63 patients' serum. Polymerase chain reaction examination at Husada Utama Hospital, Surabaya, revealed that 21 patients were confirmed positive for COVID-19 with positive PCR results, and 42 patients were healthy with negative COVID-19 results. The results showed that IgM had a diagnostic sensitivity of 85.7%, diagnostic specificity of 92.8%, a positive predictive value of 85.7%, a negative predictive value of 92.8%, and accuracy of 90.4%. In comparison, IgG had a diagnostic sensitivity of 90.4%, diagnostic specificity of 90.4%, a positive predictive value of 82.6%, a negative predictive value of 90.5%, and accuracy of 90.4%. In conclusion, IgG has a higher sensitivity than IgM, while IgM had higher specificity, positive predictive value, and negative predictive value than IgG. However, the positive, negative predictive value and efficiency values were the same forlgM and IgG.
AB - In December 2019, an outbreak of acute pneumonia occurred in Wuhan, China. The disease was transmitted between humans through droplets (coughing or sneezing) of infected patients, causing this outbreak to spread rapidly in various countries in the world, including Indonesia. On February 11, 2020, WHO announced the pneumonia was caused by Coronavirus Disease 2019 (COVID-19), which was caused by a new type of Coronavirus, the SARS-CoV-2. A rapid and accurate diagnosis is critical for the control of the COVID-19 outbreak. The widely used test is a serology-based test that detects the presence of SARS-CoV-2 IgM/lgG antibodies in the patient's body. One of the methods used for this test is Chemiluminescent Immunoassay (CUA). This study aimed to determine the reliability of CUA. The study was conducted from August to September 2020. The number of samples was 63 patients' serum. Polymerase chain reaction examination at Husada Utama Hospital, Surabaya, revealed that 21 patients were confirmed positive for COVID-19 with positive PCR results, and 42 patients were healthy with negative COVID-19 results. The results showed that IgM had a diagnostic sensitivity of 85.7%, diagnostic specificity of 92.8%, a positive predictive value of 85.7%, a negative predictive value of 92.8%, and accuracy of 90.4%. In comparison, IgG had a diagnostic sensitivity of 90.4%, diagnostic specificity of 90.4%, a positive predictive value of 82.6%, a negative predictive value of 90.5%, and accuracy of 90.4%. In conclusion, IgG has a higher sensitivity than IgM, while IgM had higher specificity, positive predictive value, and negative predictive value than IgG. However, the positive, negative predictive value and efficiency values were the same forlgM and IgG.
KW - Antibody
KW - CUA
KW - IgG
KW - IgM
UR - http://www.scopus.com/inward/record.url?scp=85189804937&partnerID=8YFLogxK
U2 - 10.24293/ijcpml.v28i1.1725
DO - 10.24293/ijcpml.v28i1.1725
M3 - Article
AN - SCOPUS:85189804937
SN - 2477-4685
VL - 28
SP - 26
EP - 30
JO - Indonesian Journal of Clinical Pathology and Medical Laboratory
JF - Indonesian Journal of Clinical Pathology and Medical Laboratory
IS - 1
ER -