This study aims to reveal the prospect of rice straw as a biosurfactant production substrate by hydrocarbonoclastic bacteria. Rice straw can be hydrolysed enzymatically into simple sugar by Penicillium sp. H9. Hydrolysis products are used as growth medium and biosurfactant production by LII61 bacteria. The concentration sugar from hydrolysis product was analyzed by Nelson method. In this study, molasses were used as a comparison substrate in biosurfactant production. The growth response of LII61 bacteria was observed by measuring the turbidity of the culture. Biosurfactant products were evaluated by measuring the emulsification activity (%) and surface tension (mN/m). Acquisition of sugar from rice straw hydrolysis product (RSHP) was 209.25 μg/mL. The optimum growth both of RSHP and molasses substrates were obtained on the 5th day of incubation with a culture turbidity value of OD λ650 0.201 and OD λ650 0.157 respectively. The lowest surface tension obtained in culture of RSHP was 48.85 mN/m. It was better than biosurfactant product on the molasses substrate on the 3rd day incubation. However, during the incubation time both substrates did not show emulsification activity. Biosurfactants produced have certain characteristics on variations in pH and temperature.
|Journal||IOP Conference Series: Earth and Environmental Science|
|Publication status||Published - 9 Jan 2019|
|Event||2nd International Conference on Collaboration Seminar of Chemistry and Industry, CoSCI 2018, in conjunction with 23rd Indonesian Society for Biochemistry and Molecular Biology, ISBMB 2018 - Surabaya, Indonesia|
Duration: 11 Oct 2018 → 12 Oct 2018
- LII61 Hydrocarbonoclastic Bacteria
- Penicillium sp. H9
- Rice Straw