Toxicity test from gloriosa superba l leaves extract in rats (rattus novegicus)

Objective: Plants Gloriosa Superba L family of Liliaceae, containing one type of alkaloid that is colchicines. Colchicine is used in the treatment of gout and rheumatism. The aims of this study were to make standardized herbal preparations by determining the effects of decreasing of uric acid level and conducting toxicity test. Methods: The determination of the effect of Gloriosa Superba L. leaf extracts, toward decrease uric acid level using a white male Wistar rats were induced by potassium oxonic dose of 250 mg/kg BW, and acute toxicity testing was conducted using a chemical substance once or multiple times within 24 hour period. Toxicity tests are designed to determine the LD₅₀ of the drug. And acute toxicity test has been carried out in the colchicine of standardized Gloriosa Superba L with a dose of 121.24 mg/kg, 363.73 mg/kg, 1091.196 mg/kg and 3273.6 mg/kg, and pure colchicine at a dose of 0.045 mg/kg, 0.45 mg/kg, 4.5 mg/kg, 45 mg/kg and 450 mg/kg using rats. Toxicity test used rats as experimental objects. Results: Data were analyzed with SPSS version 20, the real difference between treatments was tested with Two Way ANOVA and gave a significant difference (significant) between the treatment of hyperuricemia with the negative control group, positive control, and the test preparation group with a significant price of each group was 0.000 (α < 0.05). The results showed that the extract of Gloriosa Superba L decreased uric acid level in the blood of male Wistar strain rats which were induced with potassium oxonic. Conclusion: Results LD₅₀ for Gloriosa Superba L leaf extract falls into the category of practical non-toxic (>1500 mg/kg BW). Obtained LD₅₀ of colchicine was 7.92 mg/kg.