TY - JOUR
T1 - Toxicity of copper pollution on sperm quality of Cyprinus carpio
AU - Pramudya, M.
AU - Hayati, A.
AU - Armando, D. S.
AU - Wulansari, E.
AU - Faridah, N.
AU - Susilo, R. J.K.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2021/4/1
Y1 - 2021/4/1
N2 - Water pollution by heavy metal waste from industry, agriculture, and other human activities affect the reproductive health of aquatic biota, especially freshwater fish. This study aimed to evaluate the quality of Cyprinus carpio sperm contaminated with copper (Cu). The sample used was gonad from mature fish. The sperm and eggs were taken by stripping, six times of repetition. Cu with a concentration of 0, 10, 25, 50, and 75 ppm were used to examine the sperm quality (duration of motility and viability, DNA fragmentation, malondialdehyde level) and the ability of sperm to fertilize. The data was collected after sperm incubation with the variation of Cu concentration for five seconds. The sperm observation was done under inverted and fluorescence microscopes. The results showed that Cu exposure caused fertilization failure, thus reducing the number of fertilized eggs. The increase of Cu concentration from 10 ppm to 25 ppm caused a decrease in sperm quality and sperm fertilization. The increase in Cu concentration also raised the percentage of DNA fragmentation.
AB - Water pollution by heavy metal waste from industry, agriculture, and other human activities affect the reproductive health of aquatic biota, especially freshwater fish. This study aimed to evaluate the quality of Cyprinus carpio sperm contaminated with copper (Cu). The sample used was gonad from mature fish. The sperm and eggs were taken by stripping, six times of repetition. Cu with a concentration of 0, 10, 25, 50, and 75 ppm were used to examine the sperm quality (duration of motility and viability, DNA fragmentation, malondialdehyde level) and the ability of sperm to fertilize. The data was collected after sperm incubation with the variation of Cu concentration for five seconds. The sperm observation was done under inverted and fluorescence microscopes. The results showed that Cu exposure caused fertilization failure, thus reducing the number of fertilized eggs. The increase of Cu concentration from 10 ppm to 25 ppm caused a decrease in sperm quality and sperm fertilization. The increase in Cu concentration also raised the percentage of DNA fragmentation.
UR - http://www.scopus.com/inward/record.url?scp=85104178539&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/718/1/012019
DO - 10.1088/1755-1315/718/1/012019
M3 - Conference article
AN - SCOPUS:85104178539
SN - 1755-1307
VL - 718
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012019
T2 - 3rd International conference on Fisheries and Marine Sciences, INCOFIMS 2020
Y2 - 10 September 2020
ER -
