2-Methoxyethanol (2-ME) is one of the plasticizer able to induce hormonal system disorders, such insulin resistance. This research was designed to determine the effect of 2-methoxyethanolon blood glucose, levels of nitrite and damage Langerhans island of mice (Mus musculus L.). This research was experimental research with Completely Randomized Design (CRD). Female mice strain Balb/C was used as an animal model. Samples were divided into 5 groups; Negative control (NC), KP Positive control (PC) injected intraperitoneally with Streptozotocin (STZ) dose of 30 mg/kg Body Weight (BW) daily for five consecutive days; Treated mice (T1, T2, T3 were injected intraperitoneally with 2-ME daily for ten consecutive days, at dose of 200 mmol/kg BW, 250 mmol/kg and 300 mmol/kg BW respectively). On 16th day and 21thday, mice were sacrificed using chloroform. Fasting blood glucose and nitric oxide (NO) levels were observed in this study. Liver tissue was processed histologically by paraffin method stained with hematoxylin eosin. NO in serum was observed using spectrophotometer with a wavelength of 540. The diameter of Langerhans islands was measured with light microscope. The result of this research was analyzed using the One Way Anova test (α=0.05) was performed to determine the effect of the treatment and Duncan test to find out the difference between the treatment groups. The result showed 2-methoxyethanol was able to increase blood glucose level, while NO was found to be decreased especially in a group treated with 200 mmol/kg BW 2-ME.