TY - JOUR
T1 - Topical instillation of resveratrol preconditioned wharton’s jelly mesenchymal stem cell secretome preserves ocular surface in experimental model of severe dry eye disease
AU - Astaridewi, Diandra
AU - Komaratih, Evelyn
AU - Rindiastuti, Yuyun
AU - Wirohadidjojo, Yohanes Widodo
AU - Legowo, Djoko
AU - Lestari, Ni Made Inten
AU - Dewi, Diandra Astari
AU - Satya, I. Made
AU - Rantam, Fedik A.
AU - Prakoeswa, Cita R.S.
N1 - Publisher Copyright:
© 2020 Diandra Astaridewi, Evelyn Komaratih, Yuyun Rindiastuti, Yohanes Widodo Wirohadidjojo, Djoko Legowo, Ni Made Inten Lestari, Diandra Astari Dewi, I. Made Satya, Fedik A. Rantam, Cita R. S. Prakoeswa.
PY - 2020/1/2
Y1 - 2020/1/2
N2 - BACKGROUND: Secretome derived from Wharton’s jelly mesenchymal stem cells (WJ-MSC) has a beneficial effect for ocular surface regeneration; however, the high amount of vascular endothelial growth factor (VEGF) remains a challenge for its application. AIM: The aim of the study was to investigate the effect of resveratrol (RV) (VEGF reducing agent) preconditioned WJ-MSC secretome in Concanavalin A-induced severe dry eye model. METHODS: Pre-and post-experimental study composed of topical instillation of WJ-MSC secretome group, balanced salt solution control group, and normal control group. Tear production, tear break-up time (TBUT), corneal fluorescein dye staining, VEGF level in aqueous tear, goblet cell density, and inflammatory cells in the ocular surface were analyzed. RESULTS: Topical instillation of RV preconditioned WJ-MSC secretome successfully improved tear film production (p = 0.008) and TBUT (p = 0.008), promoted goblet cell restoration (p = 0.023) and reduced corneal fluorescein staining (p = 0.003), while inhibited infiltration of inflammatory cells (p = 0.01) and secretion of VEGF in aqueous tear (p = 0.003). CONCLUSION: Topical instillation of RV preconditioned WJ-MSC secretome has great potential as cell-free based therapy to preserve ocular surface in an experimental model of severe Dry eye disease.
AB - BACKGROUND: Secretome derived from Wharton’s jelly mesenchymal stem cells (WJ-MSC) has a beneficial effect for ocular surface regeneration; however, the high amount of vascular endothelial growth factor (VEGF) remains a challenge for its application. AIM: The aim of the study was to investigate the effect of resveratrol (RV) (VEGF reducing agent) preconditioned WJ-MSC secretome in Concanavalin A-induced severe dry eye model. METHODS: Pre-and post-experimental study composed of topical instillation of WJ-MSC secretome group, balanced salt solution control group, and normal control group. Tear production, tear break-up time (TBUT), corneal fluorescein dye staining, VEGF level in aqueous tear, goblet cell density, and inflammatory cells in the ocular surface were analyzed. RESULTS: Topical instillation of RV preconditioned WJ-MSC secretome successfully improved tear film production (p = 0.008) and TBUT (p = 0.008), promoted goblet cell restoration (p = 0.023) and reduced corneal fluorescein staining (p = 0.003), while inhibited infiltration of inflammatory cells (p = 0.01) and secretion of VEGF in aqueous tear (p = 0.003). CONCLUSION: Topical instillation of RV preconditioned WJ-MSC secretome has great potential as cell-free based therapy to preserve ocular surface in an experimental model of severe Dry eye disease.
KW - Dry eye disease
KW - Mesenchymal stem cell
KW - Ocular surface
KW - Resveratrol
KW - Secretome
UR - http://www.scopus.com/inward/record.url?scp=85097388181&partnerID=8YFLogxK
U2 - 10.3889/oamjms.2020.5305
DO - 10.3889/oamjms.2020.5305
M3 - Article
AN - SCOPUS:85097388181
SN - 1857-5749
VL - 8
SP - 1116
EP - 1123
JO - Open Access Macedonian Journal of Medical Sciences
JF - Open Access Macedonian Journal of Medical Sciences
IS - B
ER -