TY - JOUR
T1 - Topical Epigallocatechin-3-gallate hydrogels regulated inflammation and pain
AU - Ismiyatin, Kun
AU - Wahluyo, Soegeng
AU - Purwanto, Bambang
AU - Soetojo, Adioro
AU - Rahayu, Retno Pudji
AU - Mukono, Indri Safitri
N1 - Funding Information:
This research was conducted with the permission of the Department of Medical Biochemistry, Faculty of Medicine, and the Faculty of Dentistry, Universitas Airlangga, Surabaya. Financial support was provided through Basic Excellence Research of Higher Education grant from Ministry of Research, Technology and Higher Education of the Republic of Indonesia year 2018.
Publisher Copyright:
© 2019 Journal of International Dental and Medical Research.
PY - 2019
Y1 - 2019
N2 - The aim of this study is to determine in vivo response of topical Epigallocatechin-3-gallate (EGCG) hydrogels to pain and inflammation through Toll-like receptor 4 (TLR4), Superoxide dismutase (SOD1), Prostaglandin E2 (PG-E2) and Transient receptor potential vanilloid 1 (TRPV1) in pulpal inflammation. Rats (n=35) were divided into a normal group (N), a positive control group induced with LPS for 6 hours (C1), a group induced with LPS for 24 hours (C2), a treatment group induced with LPS and topical EGCG 120 μg/ml (T1) for 6 hours and a group induced with LPS and topical EGCG 120 μg/ml for 24 hours (T2). Tissues were collected and observed by means of immunohistochemistry (IHC) to detect the expression of TLR4, SOD1, PG-E2 and TRPV1. Data were analyzed using a One-Way ANOVA test for TLR4, PG-E2 and TRPV1, while SOD1 was analyzed using a Brown-Forsythe test and Games Howell test. Application of topical 120 μg/ml EGCG hydrogels in tooth cavities at 6 hours and 24 hours pulpal inflammation significantly increased the expression of SOD1, while significantly decreasing the expressions of TLR4, PG-E2 and TRPV1 (P < 0.001). EGCG can be used as a pain inhibitor, anti-inflammatory agent and antioxidant agent against pulpal inflammation in rat models.
AB - The aim of this study is to determine in vivo response of topical Epigallocatechin-3-gallate (EGCG) hydrogels to pain and inflammation through Toll-like receptor 4 (TLR4), Superoxide dismutase (SOD1), Prostaglandin E2 (PG-E2) and Transient receptor potential vanilloid 1 (TRPV1) in pulpal inflammation. Rats (n=35) were divided into a normal group (N), a positive control group induced with LPS for 6 hours (C1), a group induced with LPS for 24 hours (C2), a treatment group induced with LPS and topical EGCG 120 μg/ml (T1) for 6 hours and a group induced with LPS and topical EGCG 120 μg/ml for 24 hours (T2). Tissues were collected and observed by means of immunohistochemistry (IHC) to detect the expression of TLR4, SOD1, PG-E2 and TRPV1. Data were analyzed using a One-Way ANOVA test for TLR4, PG-E2 and TRPV1, while SOD1 was analyzed using a Brown-Forsythe test and Games Howell test. Application of topical 120 μg/ml EGCG hydrogels in tooth cavities at 6 hours and 24 hours pulpal inflammation significantly increased the expression of SOD1, while significantly decreasing the expressions of TLR4, PG-E2 and TRPV1 (P < 0.001). EGCG can be used as a pain inhibitor, anti-inflammatory agent and antioxidant agent against pulpal inflammation in rat models.
KW - PG-E2
KW - SOD1
KW - TLR4
KW - Topical EGCG
KW - TRPV1
UR - http://www.scopus.com/inward/record.url?scp=85069536048&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85069536048
SN - 1309-100X
VL - 12
SP - 54
EP - 60
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 1
ER -