Three models of primary dysmenorrhea via estradiolbenzoate and oxytocin induction: an experimental study

Objective: To evaluate the PGF2 profile and its association with symptoms in several models of primary dysmenorrhea. Methodogy: Forty Sprague Dawley rats were placed into four groups, each with ten rats: control, primary dysmenorrhea 1 (PD1), primary dysmenorrhea 2 (PD2), and primary dysmenorrhea 3 (PD3). The pain as main dysmenorrhea symptom was assessed by observing writhin glatency and frequency. The enzyme linked immunosorbent assay method was used to evaluate PGF2 levels in serum. Using hematoxyline eosin staining, the uterus was examined histopathologically. Results: The PD3 model had writhing characteristics, PGF2 levels, and degree of inflammation that are comparable to the PD2 model. Conclusion: Our proposed PD model has clinical and biomolecular and histopathological characteristics comparable to existing models. Thus, this new model can be an alternative for modeling primary dysmenorrhea.