TY - GEN
T1 - The Viability Differences on Oocyte Vitrification Surrounded by Cumulus Cells and Oocyte Vitrification not Surrounded by Cumulus Cells after in Vitro Maturation
AU - Nur Kasman, Aa Muhammad
AU - Santoso, Budi
AU - Widjiati, Widjiati
AU - Hinting, Aucky
AU - Wajan Tirthaningsih, Ni
AU - I'Tishom, Reny
AU - Utomo, Budi
AU - Sasmito Djati, Mochammad
N1 - Publisher Copyright:
© 2020 ACM.
PY - 2020/11/6
Y1 - 2020/11/6
N2 - This study aims to evaluate the viability differences on oocyte vitrification surrounded by cumulus cells and oocyte vitrification not surrounded by cumulus cells after in vitro maturation. The oocyte collection was through the aspiration technique of puncturing the 10 cc disposable syringe with 18 G needle filled with oocyte medium on follicle 2-6mm. Oocytes with intact cytoplasm were chosen and divided into two treatment groups; oocytes surrounded by cumulus cells and not surrounded by cumulus cells. The oocyte vitrification used hemi-straw into liquid nitrogen for ten days and continued with warming. In vitro maturation, each was with 50 μl drop for 5-10 oocytes covered with mineral oil in incubator for 22 hours under environmental condition of 5% CO2 , temperature of 38 °C, and humidity of 95-99%. This study resulted in the morphological proportion of normal oocytes was 83.3% in the oocyte group surrounded by cumulus cells and 70% in the oocyte group not surrounded by cumulus cells. The oocyte viability on groups of oocyte surrounded by cumulus cells was insignificantly higher compared to the groups of oocyte not surrounded by cumulus cells after the vitrification and warming followed by in vitro maturation.
AB - This study aims to evaluate the viability differences on oocyte vitrification surrounded by cumulus cells and oocyte vitrification not surrounded by cumulus cells after in vitro maturation. The oocyte collection was through the aspiration technique of puncturing the 10 cc disposable syringe with 18 G needle filled with oocyte medium on follicle 2-6mm. Oocytes with intact cytoplasm were chosen and divided into two treatment groups; oocytes surrounded by cumulus cells and not surrounded by cumulus cells. The oocyte vitrification used hemi-straw into liquid nitrogen for ten days and continued with warming. In vitro maturation, each was with 50 μl drop for 5-10 oocytes covered with mineral oil in incubator for 22 hours under environmental condition of 5% CO2 , temperature of 38 °C, and humidity of 95-99%. This study resulted in the morphological proportion of normal oocytes was 83.3% in the oocyte group surrounded by cumulus cells and 70% in the oocyte group not surrounded by cumulus cells. The oocyte viability on groups of oocyte surrounded by cumulus cells was insignificantly higher compared to the groups of oocyte not surrounded by cumulus cells after the vitrification and warming followed by in vitro maturation.
KW - In vitro maturation
KW - Oocyte
KW - cumulus cells
KW - viability
KW - vitrification
UR - http://www.scopus.com/inward/record.url?scp=85103835930&partnerID=8YFLogxK
U2 - 10.1145/3444884.3444912
DO - 10.1145/3444884.3444912
M3 - Conference contribution
AN - SCOPUS:85103835930
T3 - ACM International Conference Proceeding Series
SP - 145
EP - 148
BT - Proceedings of 2020 7th International Conference on Biomedical and Bioinformatics Engineering, ICBBE 2020
PB - Association for Computing Machinery
T2 - 7th International Conference on Biomedical and Bioinformatics Engineering, ICBBE 2020
Y2 - 6 November 2020 through 9 November 2020
ER -