TY - JOUR
T1 - The validation of the Helicobacter pylori CagA typing by immunohistochemistry
T2 - nationwide application in Indonesia
AU - Miftahussurur, Muhammad
AU - Doohan, Dalla
AU - Syam, Ari Fahrial
AU - Nusi, Iswan Abbas
AU - Waskito, Langgeng Agung
AU - Fauzia, Kartika Afrida
AU - Rezkitha, Yudith Annisa Ayu
AU - Dewayani, Astri
AU - I'tishom, Reny
AU - Maulahela, Hasan
AU - Uchida, Tomohisa
AU - Yamaoka, Yoshio
N1 - Funding Information:
This report is based on work supported in part by grants from the Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan ( 15H02657 , 16H05191 , 16H06279 , 18KK0266 , and 19H03473 ) (YY) and the National Institutes of Health ( DK62813 ) (YY). This work was also supported by the Japan Society for the Promotion of Science (JSPS) Institutional Program for Core-to-Core Program; B. Asia-Africa Science Platform (YY). LAW, DD, and KAF are PhD students supported by the Japanese Government (MEXT) scholarship program for 2015, 2016, and 2017, respectively.
Publisher Copyright:
© 2020 Elsevier GmbH
PY - 2020/9
Y1 - 2020/9
N2 - We aimed to validate 2 types of antibodies, anti-CagA antibody and anti-East Asian CagA specific antibody (α-EAS antibody) for the determination of CagA status in Indonesia. We also confirmed the performance of α-EAS antibody for the detection of East Asian-type CagA H. pylori. Immunohistochemistry was performed using anti-CagA antibody and α-EAS antibody on gastric biopsy specimens from a total of 967 Indonesian patients. Diagnostic values of immunohistochemistry were evaluated with PCR-based sequencing as gold standard. Anti-CagA antibody had high sensitivity, specificity, and accuracy (87.0 %, 100 %, and 98.8 %, respectively) for determining CagA status. The α-EAS antibody was not suitable for the purpose of CagA status determination, as it had a low sensitivity (23.9 %). High specificity (97.6 %) but low sensitivity (41.2 %) and accuracy (66.3 %) was observed in α-EAS antibody to detect East Asian-type CagA. Patients with positive result of immunohistochemistry using anti-CagA antibody had significantly higher monocyte infiltration score in antrum (P < 0.001) and corpus (P = 0.009). In conclusion, the anti-CagA antibody is still suitable to be used in Indonesia for determining the CagA status, whilst the α-EAS antibody was not appropriate to discriminate between East Asian-type and non-East Asian-type CagA in Indonesia.
AB - We aimed to validate 2 types of antibodies, anti-CagA antibody and anti-East Asian CagA specific antibody (α-EAS antibody) for the determination of CagA status in Indonesia. We also confirmed the performance of α-EAS antibody for the detection of East Asian-type CagA H. pylori. Immunohistochemistry was performed using anti-CagA antibody and α-EAS antibody on gastric biopsy specimens from a total of 967 Indonesian patients. Diagnostic values of immunohistochemistry were evaluated with PCR-based sequencing as gold standard. Anti-CagA antibody had high sensitivity, specificity, and accuracy (87.0 %, 100 %, and 98.8 %, respectively) for determining CagA status. The α-EAS antibody was not suitable for the purpose of CagA status determination, as it had a low sensitivity (23.9 %). High specificity (97.6 %) but low sensitivity (41.2 %) and accuracy (66.3 %) was observed in α-EAS antibody to detect East Asian-type CagA. Patients with positive result of immunohistochemistry using anti-CagA antibody had significantly higher monocyte infiltration score in antrum (P < 0.001) and corpus (P = 0.009). In conclusion, the anti-CagA antibody is still suitable to be used in Indonesia for determining the CagA status, whilst the α-EAS antibody was not appropriate to discriminate between East Asian-type and non-East Asian-type CagA in Indonesia.
KW - Anti-East Asian CagA antibody
KW - CagA typing
KW - Immunohistochemistry
KW - Infectious disease
KW - anti-CagA antibody
UR - http://www.scopus.com/inward/record.url?scp=85088113639&partnerID=8YFLogxK
U2 - 10.1016/j.acthis.2020.151594
DO - 10.1016/j.acthis.2020.151594
M3 - Article
C2 - 32778248
AN - SCOPUS:85088113639
SN - 0065-1281
VL - 122
JO - Acta Histochemica
JF - Acta Histochemica
IS - 6
M1 - 151594
ER -
