The use of human dried amniotic membrane (H-DAM) as a biomaterial patch for wound healing of gastric perforation viewed from the fgf and vegf

Gastric perforation in neonates is a medical emergency with a 75% mortality rate. Several studies have been conducted to promote tissue healing, including using various surgical procedures and materials, including H-DAM technology. To compare the expression of Fibroblast Growth Factor (FGF) and Vascular Endothelial Growth Factor (VEGF) in gastric perforation repair employing H-DAM as a patch biomaterial to that of an omental patch in New Zealand white rabbits. This experiment was carried out on a New Zealand white rabbit model divided into three groups: amniotic membrane, omental patch, and primary repair. An incision was made wide with a depth of the entire gastric wall in the gastric corpus and the perforation repair was carried out. The repair is closed with the H-DAM in the amniotic group and using the omentum in the omental group. The expression of FGF and VEGF was used to evaluate the wound healing process. VEGF and FGF expression were higher in gastric perforation models sutured with H-DAM than in models without H-DAM. There were significant differences in the average expression of VEGF and FGF. Human-dried amnion has a role in the wound-healing process in gastric perforation repair models, hence H-DAM may be the a preferred repair strategy for gastric perforation.