The use of displacement loop mtDNA in halal forensic investigation in Indonesia

Background: Indonesia, home to the largest Muslim population in the world, is a major potential market for halal products, yet one which is subject to frequent adulteration of imported food items. The application of halal forensic scientific methods to detect adulteration is essential in determining the halal quality of food content. Purpose: The purpose of this study was to evaluate the DNA content of molecular-based food products presumed to having been mixed in order to determine their halal quality. Materials and Methods: The research was descriptional in character using the D-Loop region for the primary design relating to six species, namely Gallus gallus (chicken), Sus sacrofa (pork), Boss Taurus (bovine), Capra hircus (goat), Equus caballus (horse), Rattus novergicus (rat) and the optimization of the cytochrom b primer as a comparative control of primary D-Loop success. Result: The study showed that the D-loop region can potentially be employed for investigative purposes within the halal forensic field as the primary cytochrom b mitochondrial DNA used for comparison. Conclusion: Primer that designed from displacement loop region and cytochrome b in mitochondria from six species can be used for determination in halal forensic as sensitive DNA amplification using PCR.