TY - JOUR
T1 - The study of live and frozen marine fish in commodities imports using conventional and the Polymerace Chain Reaction (PCR)
AU - Sukmawati, A. R.
AU - Pursetyo, K. T.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2021/4/1
Y1 - 2021/4/1
N2 - Indonesia has big potency of fishery which has entered in export-import market. That export-import activitiy isn't balance with quality warranty and the quality assurance. The quality warranty and the quality assurance are important because there found bacteria which have zoonotic characteristic. The method of data collection consisted of primary data and secondary data. Data collection was carried out with active participation, observation, interview and literature. Conventional method was used for bacterial examination on a live fish. The use of this method started from the initial isolation, purification, base test, biochemical test and the last was identification process. Bacteria found in a live fish by using the conventional method are Pasteurella haemolytica, Acinetobacter sp, Aeromonas hydrophila, Pseudomonna maltophilia, Pseudomonas sp, Vibrio sp, Pseudomonas mallei, Plesiomonas sp, Alcaligenes sp and Pseudomonas aeruginosa. Polymerace Chain Reaction (PCR) method was used for bacterial examination in frozen fish. This method had many steps, there were extraction, amplification, electrophoresis, and the last was reading the result with UVdoc machine. Primer which was provided that was used to diagnostic DNA bacteria of Aeromonas salmonicida and Edwardsiella tarda. During the study no bacteria of Aeromonas salmonicida and Edwardsiella tarda found, in frozen fish by PCR method.
AB - Indonesia has big potency of fishery which has entered in export-import market. That export-import activitiy isn't balance with quality warranty and the quality assurance. The quality warranty and the quality assurance are important because there found bacteria which have zoonotic characteristic. The method of data collection consisted of primary data and secondary data. Data collection was carried out with active participation, observation, interview and literature. Conventional method was used for bacterial examination on a live fish. The use of this method started from the initial isolation, purification, base test, biochemical test and the last was identification process. Bacteria found in a live fish by using the conventional method are Pasteurella haemolytica, Acinetobacter sp, Aeromonas hydrophila, Pseudomonna maltophilia, Pseudomonas sp, Vibrio sp, Pseudomonas mallei, Plesiomonas sp, Alcaligenes sp and Pseudomonas aeruginosa. Polymerace Chain Reaction (PCR) method was used for bacterial examination in frozen fish. This method had many steps, there were extraction, amplification, electrophoresis, and the last was reading the result with UVdoc machine. Primer which was provided that was used to diagnostic DNA bacteria of Aeromonas salmonicida and Edwardsiella tarda. During the study no bacteria of Aeromonas salmonicida and Edwardsiella tarda found, in frozen fish by PCR method.
UR - http://www.scopus.com/inward/record.url?scp=85104191653&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/718/1/012023
DO - 10.1088/1755-1315/718/1/012023
M3 - Conference article
AN - SCOPUS:85104191653
SN - 1755-1307
VL - 718
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012023
T2 - 3rd International conference on Fisheries and Marine Sciences, INCOFIMS 2020
Y2 - 10 September 2020
ER -