The Stemness and Quiescence Maintenance of Human Iliac Bone Marrow Mesenchymal Stem Cells by Resveratrol: An In Vitro Study

Abstract Resveratrol is one of the active substances in grape seeds that may maintain the stemness and quiescence of MSCs. The aim of this study was to investigate the effect of Resveratrol administration in maintaining the human iliac bone marrow stem cells (hIBM-MSCs) stemness and quiescence. The examination processes in passage 3 of hIBM-MSCs for Cluster of Differentiation (CD)14, CD34, CD44, CD45, CD73, and CD105 expressions were done by using a method called flow cytometry. This study divided the samples into four experimental groups; they were a control group (hIBM-MSCs only), a group of hIBM-MSCs with 0.1μg Resveratrol and a group of hIBM-MSCs with 1μg Resveratrol. Moreover, SOX2 expression in passage 3 and 5 of hIBM-MSCs was examined by applying RT-qPCR. Meanwhile, cdk, p21 and pRb expression in passage 3 and 6 of hIBM-MSCs were investigated by employing of Immunocytochemistry. The proliferation rate of hIBM-MSCs in passage 3 and 6 between control and treatment groups were examined. Data were then analyzed by applying the analysis of variance continued with post hoc test (p<0.05). This investigation revealed that hIBM-MSCs were positively expressed by CD44, CD73, CD105 and CD105 but not with CD13, CD34, and CD45 expression. The proliferation rates in hIBM-MSCs were significantly different between passage 3 and 6 in resveratrol groups compared to control group (p<0.05). Meanwhile, Sox expression was fundamentally enhanced in treatment groups after resveratrol administration in contrast to the control group, especially in passage 3 and 5 (p<0.05). Additionally, the expressions of cdk, p21 and pRb were significantly improved in resveratrol groups compared to the control groups in passage 3 and 6 (p<0.05)