The stability and irritability study of the chitosan-Aloe vera spray gel as wound healing

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8 Citations (Scopus)


Chitosan is a natural polysaccharide widely used in various clinical applications including regeneration of skin tissue. Aloe vera has properties in healing burns on the skin, anti-inflammatory effect, and leaves a protective layer on the skin after drying so it provides protection to the wound. The spray gel of chitosan-A. vera was developed as a wound healing that has combined of effect of both component and easy to use. The purpose of this study was to determine the physical stability and irritability of chitosan-A. vera spray gel. The spray gel stability test was conducted using thermal cycling and centrifugation methods. The organoleptic, viscosity, and pH of the spray were evaluated. The irritation test was performed by Draize Rabbit Test method. Chitosan (0.5%)-A. vera (1%) spray gel characteristics has a weak yellow color, clear, and a strong A. vera odor. The pH of the spray gel was 4.88 ± 0.01; and the viscosity was 36.50 ± 0.23 cps. The result from the chitosan (0.5%)-A. vera (1%) spray gel stability test using thermal cycling method showed a decrease of viscosity, but remained stable when evaluated using centrifugation method. There was no difference in the pH and organoleptic observation from both tests. Based on the scoring and analysis of the reaction in rabbit skin, the Primary Irritation Index (PII) obtained was 0.56. The spray gel of chitosan (0.5%)-A. vera (1%) was stable and according to response category from the acute dermal irritation test, it can be concluded that chitosan (0.5%)-A. vera (1%) spray gel had a slightly irritating effect.

Original languageEnglish
Pages (from-to)651-656
Number of pages6
JournalJournal of Basic and Clinical Physiology and Pharmacology
Issue number4
Publication statusPublished - 1 Jul 2021


  • Aloe vera
  • chitosan
  • irritability study
  • stability


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