TY - JOUR
T1 - The squamous cell carcinoma cell line OM-1 retains both p75-dependent stratified epithelial progenitor potential and cancer stem cell properties
AU - Uetsuki, Ryo
AU - Higashikawa, Koichiro
AU - Okuda, Satoshi
AU - Yamakado, Nao
AU - Ishida, Fumi
AU - Rizqiawan, Andra
AU - Ono, Shigehiro
AU - Takechi, Masaaki
AU - Mizuta, Kuniko
AU - Shigeishi, Hideo
AU - Kamata, Nobuyuki
AU - Tobiume, Kei
N1 - Funding Information:
We are always grateful to our dear Drs. Nobuyuki Kamata and Koichiro Higashikawa, who passed away after we had established a research group in Hiroshima. We would also like to thank our colleagues at the Department of Oral and Maxillofacial Surgery for encouragement in accomplishing the study. This work was carried out at the Analysis Center of Life Science, Hiroshima University. This work was supported by KAKENHI JP19K10309 , JP19K10358 , JP19K19161 , JP18K17198 , and JP20K10139 by a Grant-in-Aid from the Japanese Ministry of Education, Culture, Sports, and Technology .
Publisher Copyright:
© 2021
PY - 2021/7
Y1 - 2021/7
N2 - The low-affinity nerve growth factor receptor p75 is a stratified epithelial stem/progenitor marker of human epithelia. We found OM-1, a human squamous cell carcinoma (SCC) cell line, showed distinct cells with p75 cluster, especially located at the center of a growing colony in a monolayer culture. A cell with p75 cluster was surrounded by cytokeratin 14- and cytokeratin 13-expressing cells that settled at the outer margin of the colony. OM-1 cells were also capable of forming tumor spheres in a cell suspension culture, an ability which was attenuated by the inhibition of p75-signaling. Intriguingly, we also found a p75-negative cell population from a growing culture of OM-1 that re-committed to become p75-clustering cells. These results indicated the possibility that SCC with epithelial multi-layering capacity can exploit the p75-dependent stratified epithelial progenitor property for the cancer stemness.
AB - The low-affinity nerve growth factor receptor p75 is a stratified epithelial stem/progenitor marker of human epithelia. We found OM-1, a human squamous cell carcinoma (SCC) cell line, showed distinct cells with p75 cluster, especially located at the center of a growing colony in a monolayer culture. A cell with p75 cluster was surrounded by cytokeratin 14- and cytokeratin 13-expressing cells that settled at the outer margin of the colony. OM-1 cells were also capable of forming tumor spheres in a cell suspension culture, an ability which was attenuated by the inhibition of p75-signaling. Intriguingly, we also found a p75-negative cell population from a growing culture of OM-1 that re-committed to become p75-clustering cells. These results indicated the possibility that SCC with epithelial multi-layering capacity can exploit the p75-dependent stratified epithelial progenitor property for the cancer stemness.
KW - Cancer stem cell (CSC)
KW - Squamous cell carcinoma (SCC)
KW - p75
UR - http://www.scopus.com/inward/record.url?scp=85106266147&partnerID=8YFLogxK
U2 - 10.1016/j.bbrep.2021.101003
DO - 10.1016/j.bbrep.2021.101003
M3 - Article
AN - SCOPUS:85106266147
SN - 2405-5808
VL - 26
JO - Biochemistry and Biophysics Reports
JF - Biochemistry and Biophysics Reports
M1 - 101003
ER -
