The role of TLR2, NF-κB, TNFα as an inflammation markers of wound dressing combination of zinc oxide with turmeric liquid extract

The aim of this study was to examine the TLR2, NF-κB, TNFa expression as an inflammation marker to evaluate the anti-inflammation properties of combination zinc oxide and turmeric rhizome liquid extract as wound dressing. Twenty three male Wistar rats, aged 3 months, weighing 175-275 grams, adapted one week before, were devided into 3 groups. Group A without any intervention as reference for normal data (n=3), Group B (excision at 3^rdday), Group C (excision at 7^th day). Group B and C contains 10 animals and has two subgroups: control (excision, n=5) and experimental group (excision with dressing application, n=5).The tissues around wound excision were removed and used to analyze histopathologically and immunohistochemistry. ANOVA was used for the statistic and regression was used for path analysis. The results showed that combination of zinc oxide and turmeric rhizome liquid extract as a dressing, were found to inhibit the inflammation, as it indicates by lowering the TLR2 at the 7th day, blocking the NF-κB expression, lowering the TNFa expression. Path analysis found a strong relationship between dressing combination zinc oxide and turmeric rhizome liquid extract to TLR2 expression (B= -0,772), NF-κB (B=0,712), TNFα (B=0,722). In conclusion, these data indicate that zinc oxide with turmeric rhizome liquid extract have beneficial effects to accelerating the inflammation through lowering TLR2, NF-κB and TNFa expressions.