The role of porphyromonas gingivalis in inducing IL-6 macrophages expression in ovaries of periodontitis models

Objective: This study aimed to determine whether P. gingivalis induction in the gingival sulcus increased IL-6 macrophages expression in the ovaries. Material and Method: This study was a laboratory experiment using a post-test-only control group design, using female white rats strain Sprague Dawley, six weeks old and in the estrous phase. They were divided into the rats without and with P. gingivalis induction groups. The rats were induced with P. gingivalis in the mesial gingival sulcus of their first right and left lower jaw molars, with 0.05 ml every three days for 19 days. On 0, 7, 14 days after bacteria induction, the animal models were euthanasia and removed their ovaries. The ovaries slides were stained by immunohistochemistry using IL-6 monoclonal antibody (diluted 1:100). The observation used light microscope in 10 fields of view with a magnification of 400x. Then, data were tested statistically with ANOVA (p<0.05). Results: The expression of IL-6 macrophages in the ovaries of the with P. gingivalis induction group was higher than in the rats with P. gingivalis induction group. However, only in the observation 14 days after the last induction, the expression of IL-6 macrophages in the ovaries of the rats with P. gingivalis induction group (12.86 ± 3.81) was significantly higher than rats without P. gingivalis induction group (5.71 ± 2.76), and was significantly higher than rats with P. gingivalis induction group after the 0 and the 7 days induction. Conclusion: EP. gingivalis promoted increased expression of IL-6 macrophages in ovaries of female periodontitis models.