TY - JOUR
T1 - The role of dexmedetomidine as brain protector assessed by cortisol, IL-6 and COX-2 concentration in rat model of traumatic brain injury
AU - Harahap, Moh Sofyan
AU - Sasongko, Himawan
AU - Bisri, Tatang
AU - Rehatta, Nancy M.
N1 - Publisher Copyright:
© 2018 Lahore Medical And Dental College. All rights reserved.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Background: Many studies over the world perform to find the best drugs for brain protection, but so far the result still inconsistent. The objective of this study is to evaluate the role of dexmedetomidine as brain protector based on cortisol, IL-6 and COX-2 plasma concentration. Methods: A laboratory experimental study with randomized control trial design had been conducted on 24 male, 3 month wistar rat, receive standard artificial brain trauma. Wistar rat divided into three group, K1 (NaCl), P (treatment) and K2 (control). Rat anesthetized with ketamin 80 mg/lgBW intra peritoneally, followed by artificial brain trauma for group K1 and P, after skin stitch group K1 receive saline intra peritoneal, and group P get dexmedetomidine 60 µgr/kgBW. K2 group as control group were not received any trauma nor medication. Dexmedetomidine or saline was given serially at 3 hours, 12 hours and 24 hours after trauma. Blood for ELISA analysis taken at pre-trauma, 12 hours and 24 hours after trauma. Result: Cortisol were unsignifficantly increased in K1, 12 hours after trauma compared to P group. IL-6 concentration in K1 increase significantly 12 and 24 hours after trauma. (160±15,57) vs (140,5±17,65) and (172,6±19,07) vs (124,2±23,6). Cox-2 concentration in K1 increase significantly 12 and 24 hours after trauma. (1491,41±341) vs (803,62±215,73) and (1048,45±170,43) vs (588,93±198,57.). Spearman’s analisys showed positive correlation between IL-6 and cortisol (ρ=0,83), IL-6 and COX-2 (ρ=0,71), COX-2 and cortisol (ρ=0,71) 12 hour after trauma. Conclusion: Dexmedetomidine has brain protection effect through decreasing of IL-6, cortisol and COX-2 concentration.
AB - Background: Many studies over the world perform to find the best drugs for brain protection, but so far the result still inconsistent. The objective of this study is to evaluate the role of dexmedetomidine as brain protector based on cortisol, IL-6 and COX-2 plasma concentration. Methods: A laboratory experimental study with randomized control trial design had been conducted on 24 male, 3 month wistar rat, receive standard artificial brain trauma. Wistar rat divided into three group, K1 (NaCl), P (treatment) and K2 (control). Rat anesthetized with ketamin 80 mg/lgBW intra peritoneally, followed by artificial brain trauma for group K1 and P, after skin stitch group K1 receive saline intra peritoneal, and group P get dexmedetomidine 60 µgr/kgBW. K2 group as control group were not received any trauma nor medication. Dexmedetomidine or saline was given serially at 3 hours, 12 hours and 24 hours after trauma. Blood for ELISA analysis taken at pre-trauma, 12 hours and 24 hours after trauma. Result: Cortisol were unsignifficantly increased in K1, 12 hours after trauma compared to P group. IL-6 concentration in K1 increase significantly 12 and 24 hours after trauma. (160±15,57) vs (140,5±17,65) and (172,6±19,07) vs (124,2±23,6). Cox-2 concentration in K1 increase significantly 12 and 24 hours after trauma. (1491,41±341) vs (803,62±215,73) and (1048,45±170,43) vs (588,93±198,57.). Spearman’s analisys showed positive correlation between IL-6 and cortisol (ρ=0,83), IL-6 and COX-2 (ρ=0,71), COX-2 and cortisol (ρ=0,71) 12 hour after trauma. Conclusion: Dexmedetomidine has brain protection effect through decreasing of IL-6, cortisol and COX-2 concentration.
KW - Brain protection
KW - COX-2
KW - Dexmedetomidine
KW - IL-6
UR - http://www.scopus.com/inward/record.url?scp=85058301512&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85058301512
SN - 1996-7195
VL - 12
SP - 1327
EP - 1333
JO - Pakistan Journal of Medical and Health Sciences
JF - Pakistan Journal of Medical and Health Sciences
IS - 3
ER -