Kacang goat is a local specialty in Indonesia. The level of oocyte maturation of Kacang goat after in vitro maturation will determine the quality of the embryo. Cumulus cells have an important role during the process of maturation for nutrient transportation. The purpose of this study was to determine the role of cumulus on the level of maturation of Kacang goat oocyte. Oocytes collected by aspiration method. The study was divided into 2 groups: the group I oocytes surrounded by more than 3 layers of cumulus. The group II was oocytes around only 1 layer of cumulus. All oocytes were matured in EBSS medium which was hormone supplemented and incubated in a 5% CO2 incubator at 38.5oC for 22 hours. Microscopic examination of oocyte maturity level was based on cumulus expansion and the presence of Polar body I. The results showed that the maturation rate in the group I was 59.26% and immature oocyte 40.74%, in group II the number of mature oocytes was 49.37% and immature oocytes 50.63 %. The conclusion of the study was that the level of oocyte maturation with thick cumulus was better than bald oocytes. Cumulus has a very important role in the maturation process of Kacang goat oocytes. The level of oocyte maturity can support embryonic development after IVF.
|Number of pages||6|
|Journal||Journal of International Dental and Medical Research|
|Publication status||Published - 2020|
- EBSS medium
- Fetal calf serum
- In vitro maturation
- Kacang goat oocyte