TY - JOUR
T1 - The protective effect of Ocimum sanctum leaf extract against lead acetate-induced nephrotoxicity and hepatotoxicity in mice (Mus musculus)
AU - Yuniarti, Wiwik Misaco
AU - Krismaharani, Nina
AU - Ciptaningsih, Priska
AU - Celia, Kristania
AU - Veteriananta, Kharisma Dwi
AU - Ma’ruf, Anwar
AU - Lukiswanto, Bambang Sektiari
N1 - Publisher Copyright:
© Copyright: Yuniarti, et al. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
PY - 2021
Y1 - 2021
N2 - Background and Aim: The liver and kidneys are the most sensitive organs to lead exposure. Drugs that inhibit the actions of lead in the liver and kidneys are required to protect them from such an exposure. This study investigates the protective effect of the leaf extract of Ocimum sanctum (OS) against lead acetate-induced nephrotoxicity and hepatotoxicity in mice. Materials and Methods: A total of 20 male mice were divided into five equal groups for the 24-day testing period. The negative control group was administered Tween-80 1% orally for 24 days. The positive control group was administered Tween-80 1% orally for 24 days and, starting on day 4, 20 mg/kg BW lead acetate orally once a day for 21 days 1 h after the administration of Tween-80 1%. The other three treatment groups were administered BW OS leaf extract orally in theamount of 140, 280, and 560 mg/kg once a day for 24 days and, starting on day 4, 20 mg/kg BW lead acetate orally for 21 days 1 h after the administration of OS leaf extract. On day 25, the mice were sacrificed to assess the levels of blood urea nitrogen (BUN), creatinine, malondialdehyde (MDA), serum glutamic-oxaloacetic transaminase (SGOT), and serum glutamic-pyruvic transaminase (SGPT) as well as the histopathological changes. Results: The OS leaf extract caused a decrease in the scores for hepatocyte degeneration and portal inflammation (p<0.05) but not for hepatic necrosis (p>0.05) in mice exposed to lead. Similar patterns were observed in the effect of OS leaf extract on the renal morphofunction. The OS leaf extract decreased the scores for hydropic degeneration, tubular necrosis, andglomerular necrosis. The levels of MDA, SGOT, SGPT, BUN, and creatinine decreased in the lead-exposed mice treated with OS leaf extract (p<0.05). Conclusion: The administration of OS leaf extract has a protective effect against lead acetate-induced hepatotoxicity and nephrotoxicity in mice.
AB - Background and Aim: The liver and kidneys are the most sensitive organs to lead exposure. Drugs that inhibit the actions of lead in the liver and kidneys are required to protect them from such an exposure. This study investigates the protective effect of the leaf extract of Ocimum sanctum (OS) against lead acetate-induced nephrotoxicity and hepatotoxicity in mice. Materials and Methods: A total of 20 male mice were divided into five equal groups for the 24-day testing period. The negative control group was administered Tween-80 1% orally for 24 days. The positive control group was administered Tween-80 1% orally for 24 days and, starting on day 4, 20 mg/kg BW lead acetate orally once a day for 21 days 1 h after the administration of Tween-80 1%. The other three treatment groups were administered BW OS leaf extract orally in theamount of 140, 280, and 560 mg/kg once a day for 24 days and, starting on day 4, 20 mg/kg BW lead acetate orally for 21 days 1 h after the administration of OS leaf extract. On day 25, the mice were sacrificed to assess the levels of blood urea nitrogen (BUN), creatinine, malondialdehyde (MDA), serum glutamic-oxaloacetic transaminase (SGOT), and serum glutamic-pyruvic transaminase (SGPT) as well as the histopathological changes. Results: The OS leaf extract caused a decrease in the scores for hepatocyte degeneration and portal inflammation (p<0.05) but not for hepatic necrosis (p>0.05) in mice exposed to lead. Similar patterns were observed in the effect of OS leaf extract on the renal morphofunction. The OS leaf extract decreased the scores for hydropic degeneration, tubular necrosis, andglomerular necrosis. The levels of MDA, SGOT, SGPT, BUN, and creatinine decreased in the lead-exposed mice treated with OS leaf extract (p<0.05). Conclusion: The administration of OS leaf extract has a protective effect against lead acetate-induced hepatotoxicity and nephrotoxicity in mice.
KW - Ocimum sanctum
KW - kidney
KW - lead acetate
KW - liver
KW - mice
UR - http://www.scopus.com/inward/record.url?scp=85101064269&partnerID=8YFLogxK
U2 - 10.14202/vetworld.2021.250-258
DO - 10.14202/vetworld.2021.250-258
M3 - Article
AN - SCOPUS:85101064269
SN - 0972-8988
VL - 14
SP - 250
EP - 258
JO - Veterinary World
JF - Veterinary World
IS - 1
ER -