The profile of crosslinked bovine hydroxyapatite gelatin chitosan scaffolds with 0.25% glutaraldehyde

Bone defect is still a major problem in dentistry. In oral cavity, trauma, congenital disorder, periodontitis, and tumor can damage bones. A biomimetic scaffold made of bovine hydroxyapatite (BHA), gelatin (GEL), and chitosan (K) (BHA-GEL-K) is on a development in recent researches. This research tends to develop BHA-GEL-K scaffolds combined with 0.25% glutaraldehyde as cross linking agent. Scaffolds' profiles were examined with FTIR, porosity size by using SEM, and compressive strength by using autograph. The adhesion of bone narrow mesenchymal stem cell (BM-MSC) to crosslinked BHA-GEL-K scaffolds with 0.25% glutaraldehyde were examined by using SEM. BHA, GEL, K and glutaraldehyde components are bonded to each other. These scaffolds have 121.21 μm of porosity and 912.86 + 83.81 kPa of compressive strength value. These scaffolds have good adhesion to BM-MSC. The adhesion of scaffolds increases since day 1 to 7. The profile of BHA-GEL-K scaffolds that crosslinked to 0.25% glutaraldehyde may fulfills a requirement of bone tissue engineering.