The potential of Eucheuma cottonii extract as a candidate for fish anesthetic agent

Red seaweed species Eucheuma cottonii was thought to have potential as an anesthetic agent because it is shown to contain compounds with bioactivity as antimicrobial, antifungal, and also anticancer. There have been no studies regarding the active ingredients of anesthetized. The objective of the study was to examine the potential of E. cottonii extract as a candidate for fish anesthetic based on toxicity, phytochemistry, and active compounds contained in the extract. The stages of research include extraction, toxicity test extracts (BSLT), phytochemical test, quantitative test compound phytochemical results, and the identification of active compounds with LC-MS. Test results showed that E. cottonii extract contains a total of phenols of 4.60 ± 0.06 mg/GAE g which is one presentation of the active ingredient in the extract. The extract had bioactivity with LC₅₀ of 221.71 ± 5.74 μg/mL. E. cottonii extract contained flavonoids compounds and saponins with a consecutive number of 24.48% and 0.22%. The results of identifying active compounds were found in several compounds that had activity asantidepressants or sedatives. E. cottonii extract can be used as a candidate for a fish anesthetic agent.