The potency of Pinus merkusii extract nanoparticles as anti Mycobacterium tuberculosis: An in vitro study

Sri Agus Sudjarwo, Giftania Wardani, Koerniasari Eraiko, Koerniasari, Ernawati

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Objective: Herbal nanoparticles have gained interest in nanomedicine, and development of new therapeutic with improved bioavailability, increased sensitivity and specificity, and reduced toxicity. The aim of this study was to evaluate the antimycobacterial activity of the Pinus merkusii extract nanoparticle in vitro. Materials and Methods: Ethanolic extract of P. merkusii was set by maceration method. Tripolyphosphate (TPP) was used to make P. merkusii nanoparticles by ionotropic gelation method. The size and morphology of the P. merkusii nanoparticle was analyzed using scanning electron microscope (SEM). The broth microdilution and micro diffusion methods were used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of P. merkusii nanoparticle on strain Mycobacterium tuberculosis H37Rv. Results: The SEM micrographs of the nanoparticle extract of P. merkusii showed that they were approximately uniform spheres with rough surface morphology and a solid dense cubical or rectangular structure. The size of P. merkusii nanoparticle ranged from10 to 800 nm; most were 500 nm. Using the broth microdilution and micro diffusion susceptibility method, P. merkusii nanoparticle was found to have the antimycobacterial effects with a MIC value of 1000 μg/ml and MBCs value of 2000 μg/ml for M.Tuberculosis H37Rv. Conclusion: P. merkusii extract nanoparticle has the lead compounds that may be developed further into antimycobacterial drugs.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalInternational Journal of Nutrition, Pharmacology, Neurological Diseases
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

Keywords

  • M.Tuberculosis H37Rv
  • Minimal bactericidal concentration
  • P. merkusii nanoparticle
  • minimal inhibitory concentration

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