The Pattern of Collagen, Col1A, BSP and MMP-8 in Alveolar Bone Socket Post Tooth Extraction of Rattus Norvegicus Strain Wistar After Induced with Hydroxyapatite Bovine Tooth Graft

Nanik Zubaidah, Yosefin Adventa, Dian Dwi Pratiwi, Latief Mooduto, Ernie Maduratna Setiawati, Sri Kunarti

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Alveolar bone defects can occur as a result of tooth extraction and also endodontic surgical procedures such as hemisection. Regenerative dentistry by using bone graft is promising in clinical settings. To explore the increase of collagen density, type I collagen (Col1A), bone sialoptrotein (BSP) and matrix metalloproteinase-8 (MMP-8) in alveolar bone socket post tooth extraction of Rattus Norvegicus strain Wistar after inducing with hydroxyapatite bovine tooth graft (HAp-BTG). Twenty-eight Wistar rats were randomly divided into 2 groups, treatment and control and 2 subgroups, day 14th and 28th. Extraction to the lower left incisor of Wistar rat was performed. The post extraction socket was filled with PEG as the control group and PEG+HAp-BTG as the treatment group. On 14th and 28th day, mandible the Wistar rats was taken. By using Masson’s Trichrome staining to examine immunology through histopathological examination, the collagen density was observed using a microscope with 400x of magnification. The Col1A, BSP and MMP-8 were examined by immunohistochemistry, and observed by a light microscope 1000x magnification. There is not significant different in collagen density between the treatment and control groups, whereas Col1A, BSP and MMP-8 were significantly different between treatment and control groups. It showed that HAp-BTG can induce the increasing of Col1A that has a pivotal role in strengthening the connective tissue in dental socket, rather than collagen in general. HAp-BTG has a potential to speed-up the recovery of dental socket post extraction and increase the strength of connective tissue by inducing Col1A.

Original languageEnglish
Pages (from-to)1447-1452
Number of pages6
JournalJournal of International Dental and Medical Research
Volume14
Issue number4
Publication statusPublished - 2021

Keywords

  • Bone regenerative
  • Bovine tooth graft
  • Collagen
  • Dental health
  • Immunology

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