The Oral Administration of Capsaicin Regulate Prostaglandin E2 and Endorphin Serum Level after 24 Hours of Orthodontic Tooth Movement in Wistar Rats (Rattus Novergicus)

Orthodontic tooth movement (OTM) pain sensation may affect the patient’s quality of life and cooperation during orthodontic treatment using fixed orthodontic appliances. This study aims to investigate the oral administration of capsaicin effect to Prostaglandin E2 and Endorphin serum level after 24 hours of OTM in vivo. 42 males, healthy, Wistar rats (Rattus novergicus), 14-16 weeks-old, 270-350 grams body weight were used as OTM animal model. Samples were randomly divided into 6 groups, namely normal control group (KN): healthy rats without OTM and treatment, a negative control group; KK1 group: application of OTM force, without olive oil administration as placebo; KK2 group: application of OTM force, with olive oil oral administration as placebo; KP1 group: application of OTM force, and oral adminsitration of capsaicin 15 mg/kg BW; KP2 group: application of OTM force, and oral adminsitration of capsaicin 30 mg/kg BW, KP3 group: application of OTM force, and oral adminsitration of capsaicin 45 mg/kg BW. PGE2 and endorphin serum levels analysis were conducted by means of ELISA. Shapiro-wilk test (p>0.05) and continued with analysis of variance (ANOVA) (p<0.05) with least significant different (LSD) or Mann-Whitney (p<0.05). The oral administration of 15 mg Capsaicin decrease significantly PGE2 serum levels. Meanwhile, 30 mg of capsaicin administration enhance significantly endorphin serum level after 24 hours of OTM in vivo. The oral administration of 15mg capsaicin can decrease significantly PGE2 serum level, while 30mg of capsaicin administration can enhance significantly the level of endorphin serum level after 24 hours of Orthodontic tooth movement in vivo.