The optimisation and standardisation of Indonesian house dust mites allergenic extract as a desensitising agent

Background: The different sequences of the allergen protease can induce different immunogenicity for the development of immunotherapy. Dermatophagoides pteronyssinus, which contain Der p1 as a major protein, will be developed as a local allergen to be used in immunotherapy for treating rhinitis allergy. Objective: This study aims to optimise and standardise the local Indonesian House Dust Mites Allergenic Extract from the harvesting into the extraction process. Method: Corn flour was used as the harvesting media by placing it under certain conditions to produce the mites. After harvesting the flour for different lengths of time, the flour was extracted and the lipid content and Der p1 assay was measured. Result: The result of the study is that the three weeks-harvesting time came higher for those parameters than the other harvesting time (p < 0.05). Conclusion: Thus, it was concluded that the standardised harvesting and extraction process had been developed to produce the standardised Indonesian House Dust Mites Allergenic Extract.