TY - JOUR
T1 - The molecular pathways of lung damage by e-cigarettes in male wistar rats
AU - Suryadinata, Rivan V.
AU - Wirjatmadi, Bambang
N1 - Publisher Copyright:
© 2021, Sultan Qaboos University. All rights reserved.
PY - 2021/8
Y1 - 2021/8
N2 - Objectives: This study aimed to analyse the stages of lung tissue damage caused by exposure to electronic cigarette (e-cigarette) smoke. The negative health effects of e-cigarettes remain debatable. Several studies have shown the adverse effects, but others opine that e-cigarettes are safer to use than their tobacco counterparts. There is, however, a possibility that the long-term health effects, such as respiratory and even kidney function impairment, are presently not obvious. The amounts of increased free radicals and pro-inflammatory cytokines from e-cigarettes result in various physiological disorders, which trigger cell damage and even cell death in the body. Methods: An experimental study was conducted between March and September 2019 in Airlangga University using a control and an experimental group of male Wistar rats to assess the levels of malondialdehyde, interleukin (IL)-8, IL-10, matrix metalloprotein-8 and type-2 collagen. The results were obtained using immunohistochemical staining methods on alveolar macrophages through Hematoxylin-Eosin staining. Results: The results showed that exposure to e-cigarette smoke caused an increase in free radicals, triggered an inflammatory process and degraded the type-2 collagen present in the lung tissue. Conclusion: Exposure to e-cigarette smoke can cause cell damage in lung tissues.
AB - Objectives: This study aimed to analyse the stages of lung tissue damage caused by exposure to electronic cigarette (e-cigarette) smoke. The negative health effects of e-cigarettes remain debatable. Several studies have shown the adverse effects, but others opine that e-cigarettes are safer to use than their tobacco counterparts. There is, however, a possibility that the long-term health effects, such as respiratory and even kidney function impairment, are presently not obvious. The amounts of increased free radicals and pro-inflammatory cytokines from e-cigarettes result in various physiological disorders, which trigger cell damage and even cell death in the body. Methods: An experimental study was conducted between March and September 2019 in Airlangga University using a control and an experimental group of male Wistar rats to assess the levels of malondialdehyde, interleukin (IL)-8, IL-10, matrix metalloprotein-8 and type-2 collagen. The results were obtained using immunohistochemical staining methods on alveolar macrophages through Hematoxylin-Eosin staining. Results: The results showed that exposure to e-cigarette smoke caused an increase in free radicals, triggered an inflammatory process and degraded the type-2 collagen present in the lung tissue. Conclusion: Exposure to e-cigarette smoke can cause cell damage in lung tissues.
KW - E-Cigarettes
KW - Eosin
KW - Hematoxylin
KW - Immunohistochemistry
KW - Lungs
UR - http://www.scopus.com/inward/record.url?scp=85112441571&partnerID=8YFLogxK
U2 - 10.18295/squmj.4.2021.003
DO - 10.18295/squmj.4.2021.003
M3 - Article
C2 - 34522410
AN - SCOPUS:85112441571
SN - 2075-051X
VL - 21
SP - 436
EP - 441
JO - Sultan Qaboos University Medical Journal
JF - Sultan Qaboos University Medical Journal
IS - 3
ER -