The mechanism of endoplasmic reticulum stress and the cyclin D2 expression on ovarian granulosa cell cycle in infertile women with endometriosis

The endometriosis related to secretory cell namely granulosa cell provides its important organelle of endoplasmic reticulum. This study aimed to analyze the influence of TNF-a against the characteristic of granulosa cell, especially the function in its organelle of endoplasmic reticulum and the cell cycle. This was an observed cross-sectional study. Samples were found by laparoscopy at the time of 17 mm sized follicle was detected. The assessment of peritoneal fluid's TNF-a level was done by ELISA, the marker of mRNA-GRP78, eIF2a, and CHOP taken from isolated granulosa cell were assessed by qPCR, and flow cytometry was used to observe cell cycle while Western blotting spent to evaluate Cyclin D2. We observed 13 women with endometriosis and 4 women with nonendometriosis. The study showed that peritoneal fluid TNF-a was higher in endometriosis group compared to non-endometriosis (48.66±3.9 vs 37.6±3.8 ng/mL, p<0.05). The study apparently indicated sharply increased of all marker's mRNA-GRP78, eIFa and CHOP with respectively 3.32, 8.54 and 1.78-fold, but Cyclin D2 also increased (97.056% vs 86.217%; p>0.05) in endometriosis women group. The granulosa cell's cycle showed the higher result on G0/G1 phase but lowering S/M phase on endometriosis group compared to control. It seemed the influence of peritoneal fluid TNF-a occurred against GRP78. Endometriosis women having higher TNF-a level suggested to lead ER stress were playing the important role in the granulosa cell cycle.