The Influence of kebar grass (biophytum petersianum k.) ethanol extract towards the amount of spermatogenic cells of mice (mus musculus) induced by 2, 3, 7, 8 tetrachlorine dibenzo-p-dioxin

The 2,3,7,8 Tetrachlorine Dibenzo-P-Dioxin (TCDD) were found as product of industrial or agricultural waste could cause abnormalities in the male reproductive organs. Kebar grass were a typical plant in Papua which were rich in Vitamin E as an antioxidant can reduce the effects of free radicals produced by TCDD. The purpose of this study was to determine the effect of ethanol extract of Kebar Grass on the number of spermatogenic cells of male mice induced TCDD. 30 male mice were divided into five groups, each group consisted of 6 replications. Group C(-) was given aquadest, group C(+) was given TCDD 0.14 μg each mice for once. T1 group was given TCDD 0.14 μg each mices for once and given Kebar grass extract 0.045mg/g BW/day. T2 group was given TCDD 0.14 μg each mices for once and given Kebar grass extract 0.080 mg/g BW/day, and T3 group was given TCDD 0,14μg each mices for once and given Kebar Grass extract 0.135mg/g BW/day. All treatment given for 53 days and testes were taken to be made as histopathology preparation to calculate the average number of spermatogenic cells. The results of this study indicate that there were significant differences (p<0.05) between group. But there's no significant differences (p>0.05) treatment of group average spermatogenia cells, administration of Kebar Grass extract can maintain the number of cells primary spermatocyte and spermatid cell counts. The treatment group with 0.135 mg/g dose showed the best dose to increase primary spermatocyte and spermatid cell counts.