The influence of ascorbic acid, creatine, and creatinine on the uric acid analysis by potentiometry using a carbon paste modified imprinting zeolite electrode

The high level of uric acid in the body is often associated with some diseases such as hyperuricemia, hypertension, renal and cardiovascular disease. Therefore early detection of the levels of uric acid is required. Spectrophotometry is the method commonly used in the medical field to determine the uric acid level. Its detection limit refers to a low uric acid level in a serum sample as the complexity of the serum matrices decreases its selectivity. The development of an imprinted zeolite based sensor to analyze uric acid by potentiometry is conducted. The carbon paste-IZ electrode shows a range of measurement of 10^-6 M - 10^-4 M, a Nernst factor of 28.2 mV decade^-1, and response time of 34 s - 44 s. The developed electrode has a high selectivity towards uric acid. Ascorbic acid, creatine, or creatinine do not interfere with uric acid analysis using the electrode suggested as the value of the selectivity coefficient (K_ij) is less than 1. The method recovery equals (113.18±14.79) % (n = 3). The developed method is compared to the spectrophotometric one and shows an accuracy of (94.47±14.64) % (n = 7).