The most common case of periodontitis is chronic periodontitis. Chronic periodontitis is characterized by the destruction of junctional epithelium caused by Porphyromonas gingivalis. Junctional epithelial attached to the tooth are mediated by laminin-5. The cleavage of the γ2 chain of laminin-5 causes junctional epithelium destruction. The aim of this study is to observe the expression of laminin-5γ2 in 1% curcumin-treated rats with periodontitis. Forty-eight periodontitis rats model were divided into 2 groups. The first is control group consisted of twenty-four periodontitis rats model exposed with Porphyromonas gingivalis. The second is treatment group consisted of twenty-four periodontitis rats model exposed to Porphyromonas gingivalis and 1% curcumin administration. Each group was observed at 24 hours on days-7 and days-14. Junctional epithelium samples in sagittal direction were examined by immunohistochemistry and were statistically analyzed using t-test (α=0.05). The result showed that laminin-5γ2 expression in treatment group was significantly lower than the control group on days-7 and days-14. In treatment group, laminin-5γ2 expression at 24 hours was significantly lower than days-7 and days-14. In treatment group, laminin-5γ2 expression did not experience a significant difference in between days-7 and days-14. This study showed that 1% curcumin can reduce laminin-5γ2 expression in periodontitis.