The increasing of vegf expression and re-epithelialization on dermal wound healing process after treatment of banana peel extract (Musa acuminata colla)

Objective: The objective of the study was to determine the efficacy of topical administration of an alcoholic bark extract of Musa acuminata Colla on cutaneous wound healing including expression of VEGF and re-epithelization on dermal in rats.

Methods: Model was performed to evaluate the excision model of wound healing activity. Full-thickness excision wounds were made on the back of rat and Musa acuminata Colla extract was topically administered (doses 25%, 50% & 75%). The formation of granulation tissue was observed on day 4, 8, 12 and 16 (post-wound) to indicate VEGF and collagen. The extract increased cellular re-epithelialisation and collagen synthesis at the wound site, shown by increase in VEGF, and total collagen content of granulation tissues. The rate of contraction in wounds was determined by tracing the wound surface onto a transparent graph paper and measuring the surface area by planimetry.

Results: From the observation in EBP, all doses were found to have wound healing activity in wound contraction and period of epithelialization. The increasing of VEGF expression was found greater in the extract treated group than control group. The tensile strength of extract treated group was increased significantly.

Conclusion: The results indicated that Musa acuminata Colla peel extracts has significant wound healing activity.