TY - JOUR
T1 - The in Vitro Inhibitory Effects of Red Pomegranate [Punica granatum Linn) Extract on Fusobacterium Nu cleat urn's and Porphyromonas Gingivalis's Growth
AU - Hernawati, Sri
AU - Soesilawati, Pratiwi
N1 - Publisher Copyright:
© 2020 EManuscript Technologies. All rights reserved.
PY - 2020/7
Y1 - 2020/7
N2 - Gram-negative anaerobic bacteria such as Fusobacterium nucleatum and Porphyromonas gingivalis are associated with halitosis since it can release the aroma of ammonia. Previous research has posited that flavonoids can inhibit bacterial growth. Red Pomegranate fruit is known to contain very effective flavonoids. Red pomegranate fruit is even known to contain polyphenols flavonoid and its derivatives and alkaloids. Based on this fact, Red pomegranate fruit is expected to be used as a mouthwash with antibacterial effect on Fusobacterium nucleatum and Porphyromonas gingivalis to control halitosis. The aim of this research was to reveal the inhibitory activity of red pomegranate extract on the growth of Fusobacterium nucleatum and Porphyromonas gingivalis by obtaining the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The red pomegranate extract was produced via ethanol extract to obtain 100% concentration of red pomegranate. Dilution was conducted in order to obtain extract in 100%, 50%, 25%, 12.5% and 6.25% concentration. Minimum Inhibitory Concentration (MIC) tests and Minimum Bactericidal Concentration (MBC) of pomegranate extracts against Fusobacterium nucleatum and Porphyromonas gingivalis bacteria was conducted to determine the in vitro inhibitory effect. Red pomegranate extract with a concentration of 50% is determined as a minimum inhibitory level and a minimum bacterial bacterial concentration against the growth of Fusobacterium nucleatum and Red pomegranate extracts with a concentration of 12.5% are determined as minimal inhibitory levels and Minimum Bactericidal Concentration against bacterial growth of Porphyromonas gingivalis. Red pomegranate extract had the potential to become an antibacterial agent toward the growth of Fusobacterium nucleatum and Porphyromonas gingivalis.
AB - Gram-negative anaerobic bacteria such as Fusobacterium nucleatum and Porphyromonas gingivalis are associated with halitosis since it can release the aroma of ammonia. Previous research has posited that flavonoids can inhibit bacterial growth. Red Pomegranate fruit is known to contain very effective flavonoids. Red pomegranate fruit is even known to contain polyphenols flavonoid and its derivatives and alkaloids. Based on this fact, Red pomegranate fruit is expected to be used as a mouthwash with antibacterial effect on Fusobacterium nucleatum and Porphyromonas gingivalis to control halitosis. The aim of this research was to reveal the inhibitory activity of red pomegranate extract on the growth of Fusobacterium nucleatum and Porphyromonas gingivalis by obtaining the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The red pomegranate extract was produced via ethanol extract to obtain 100% concentration of red pomegranate. Dilution was conducted in order to obtain extract in 100%, 50%, 25%, 12.5% and 6.25% concentration. Minimum Inhibitory Concentration (MIC) tests and Minimum Bactericidal Concentration (MBC) of pomegranate extracts against Fusobacterium nucleatum and Porphyromonas gingivalis bacteria was conducted to determine the in vitro inhibitory effect. Red pomegranate extract with a concentration of 50% is determined as a minimum inhibitory level and a minimum bacterial bacterial concentration against the growth of Fusobacterium nucleatum and Red pomegranate extracts with a concentration of 12.5% are determined as minimal inhibitory levels and Minimum Bactericidal Concentration against bacterial growth of Porphyromonas gingivalis. Red pomegranate extract had the potential to become an antibacterial agent toward the growth of Fusobacterium nucleatum and Porphyromonas gingivalis.
KW - Fusobacterium nucleatum
KW - MBC
KW - MIC
KW - Porphyromonas gingivalis
KW - Red pomegranate extract
UR - http://www.scopus.com/inward/record.url?scp=85090341628&partnerID=8YFLogxK
U2 - 10.31838/srp.2020.6.134
DO - 10.31838/srp.2020.6.134
M3 - Article
AN - SCOPUS:85090341628
SN - 0975-8453
VL - 11
SP - 954
EP - 959
JO - Systematic Reviews in Pharmacy
JF - Systematic Reviews in Pharmacy
IS - 6
ER -