TY - JOUR
T1 - The GADD45 and Wild p53 expressions resulting from moderate swimming exercise on mus musculus injected by benzopyrene
AU - Irmawati, Anis
AU - Muljono, Harjanto Joso
AU - Ketut Sudiana, I.
N1 - Publisher Copyright:
© 2019, Journal of International Dental and Medical Research.
PY - 2019
Y1 - 2019
N2 - Cancer prevalence is increasing nowadays. In Indonesia, cancer prevalence reaches1.4% out of 1,000 people. Cancer is mainly caused by two factors; genetic and environment, including a carcinogenic agent known as benzopyrene. Physical exercises can decrease the levels of blood glucose and fatty acid within the blood; can increase expression of Bax and caspase-3; but its effect on GADD45 and wild p53 expression, is still unknown. This study aimed to determine the expressions of GADD45 and wild p53 resulting from moderate swimming exercise on Mus musculus injected by benzopyrene. 18 mice were divided into three study groups; control group 1 (K1) which was not treated with any physical exercise and benzopyrene; control group 2 (K2) which was not treated with any physical exercise but induced with 0.08 mg of benzopyrene; and treated group (K3) which wastreated with physical exercise in moderate intensity and induced with 0.08 mg of benzopyrene. The buccal mucosa tissue samples were taken and stained for immunohistochemistry to be further examined under a light microscope at 400x magnification with 10 different angles. Result: There was no difference inGADD45 expression between the K1, K2, and K3 groups (p = 0.611), with a significant difference of wild p53 expression (p = 0.000). Moderate swimming exercise has no effect on GADD45 expression, but could increase wild p53 expression on Mus musculus injected by benzopyrene.
AB - Cancer prevalence is increasing nowadays. In Indonesia, cancer prevalence reaches1.4% out of 1,000 people. Cancer is mainly caused by two factors; genetic and environment, including a carcinogenic agent known as benzopyrene. Physical exercises can decrease the levels of blood glucose and fatty acid within the blood; can increase expression of Bax and caspase-3; but its effect on GADD45 and wild p53 expression, is still unknown. This study aimed to determine the expressions of GADD45 and wild p53 resulting from moderate swimming exercise on Mus musculus injected by benzopyrene. 18 mice were divided into three study groups; control group 1 (K1) which was not treated with any physical exercise and benzopyrene; control group 2 (K2) which was not treated with any physical exercise but induced with 0.08 mg of benzopyrene; and treated group (K3) which wastreated with physical exercise in moderate intensity and induced with 0.08 mg of benzopyrene. The buccal mucosa tissue samples were taken and stained for immunohistochemistry to be further examined under a light microscope at 400x magnification with 10 different angles. Result: There was no difference inGADD45 expression between the K1, K2, and K3 groups (p = 0.611), with a significant difference of wild p53 expression (p = 0.000). Moderate swimming exercise has no effect on GADD45 expression, but could increase wild p53 expression on Mus musculus injected by benzopyrene.
KW - Epithelial cells
KW - Exercise
KW - Squamous cel,. DNA damage P53
UR - http://www.scopus.com/inward/record.url?scp=85075382941&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85075382941
SN - 1309-100X
VL - 12
SP - 964
EP - 968
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 3
ER -