Purpose: The purpose of the research was to quantify the expressions of substance P (SP) in sensory nerve cells contained in the dental pulp of Wistar rats following the application of Escherichia coli lipopolysaccharide (LPS). Materials and Methods: Forty-Two male Wistar rats were divided into six groups (n = 7): Groups C1 and C2 received no treatment and served as negative controls; Groups C3 and C4 only had cavity preparation; and Groups T1 and T2 received cavity preparation and treated with LPS. All teeth were subjected to immunohistochemistry analysis of SP expression at 24 h (C1, C3, and T1) and at 72 h (C2, C4, and T2). Results: There was a significant increase in the SP of the T1 group compared to the C1 and C3 groups and of the T2 group compared to the C2 and C4 groups. While there was no significant difference in the expression of SP between the C1 and C2 groups, a significant increase was found in T1 group compared to that of the T2 group and in the C3 group compared to the C4 group (P < 0.05). Conclusion: The application of LPS to teeth resulted in the highest expression of SP after 24 h which then decreased after 72 h.

Original languageEnglish
Pages (from-to)169-173
Number of pages5
JournalSaudi Endodontic Journal
Issue number3
Publication statusPublished - 1 Sept 2019


  • Lipopolysaccharides
  • Wistar rats
  • pulpitis
  • sensory neurons
  • substance P


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