TY - JOUR
T1 - The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring
AU - Meles, Dewa Ketut
AU - Mustofa, Imam
AU - Hariadi, Mas'ud
AU - Wurlina, Wurlina
AU - Susilowati, Suherni
AU - Amaliya, Anny
AU - Suparto, Suparto
AU - Rimayanti, Rimayanti
N1 - Funding Information:
This study was supported by funding from the Directorate of Research and Community Service, Ministry of Research and Technology/National Research and Innovation Agency, Contract Number: 004/SP2H/PPM/DRPM/2020. The authors are thankful to technicians of The Singosari National AI Center, and veterinary paramedic of Gunungrejo Makmur Livestock Cooperative, for technical support. The authors are thankful to the Center for Journal Development and Scientific Publication of Airlangga University and Enago English Editing for proofreading the manuscript.
Publisher Copyright:
Copyright: Meles, et al.
PY - 2022/1
Y1 - 2022/1
N2 - Background and Aim: The production of male calf beef cattle is an agricultural innovation needed to increase the farm’s productivity as a provider of meat sources. This study aimed to determine the sex ratio of the offspring of cows inseminated with Y-bearing sperm enriched by Percoll density gradient centrifugation and swim-up, combined with delayed fixed-time artificial insemination (FTAI). Materials and Methods: Ejaculates of Simmental bulls were divided into four equal portions and grouped as T0 (control, non-sexed semen), T1 and T2 were sexed semen using Percoll density gradient centrifugation three and five levels, respectively, and T3 was sexed semen using swim-up. After the sex was sorted, the semen was diluted in a tris-egg yolk extender, packaged in French mini-straws containing 50 million live sperm cells, and frozen. Pre-sexed, post-sexed, and post-thawed spermatozoa were evaluated based on progressive motility, viability, intact plasma membrane, and abnormality. The post-thawed semen of T0 was artificially inseminated to recipient cows at 12 h after onset of estrus (not delayed FTAI). Meanwhile, the delayed FTAI was conducted 18-20 h after onset of estrus using the T0, the best of T1 and T2, and the T3 post-thawed semen. Results: The Percoll density gradient centrifugation reduced motility, viability, and intact plasma membrane but increased sperm abnormalities. Meanwhile, the swim-up process increased motility, viability, and intact plasma membrane of sperm cells but decreased sperm abnormalities. Post-thawed semen decreased motility, viability, and intact plasma membrane of sperm cells but increased sperm abnormalities. The sex ratio of the Simmental crossbred offspring was 96.08% and 100% in T1 and T3, respectively, compared to 48.25% and 67.39% in T0 not delayed and delayed FTAI, respectively. Conclusion: The Percoll density gradient centrifugation and swim-up methods are prospective for obtaining male offspring.
AB - Background and Aim: The production of male calf beef cattle is an agricultural innovation needed to increase the farm’s productivity as a provider of meat sources. This study aimed to determine the sex ratio of the offspring of cows inseminated with Y-bearing sperm enriched by Percoll density gradient centrifugation and swim-up, combined with delayed fixed-time artificial insemination (FTAI). Materials and Methods: Ejaculates of Simmental bulls were divided into four equal portions and grouped as T0 (control, non-sexed semen), T1 and T2 were sexed semen using Percoll density gradient centrifugation three and five levels, respectively, and T3 was sexed semen using swim-up. After the sex was sorted, the semen was diluted in a tris-egg yolk extender, packaged in French mini-straws containing 50 million live sperm cells, and frozen. Pre-sexed, post-sexed, and post-thawed spermatozoa were evaluated based on progressive motility, viability, intact plasma membrane, and abnormality. The post-thawed semen of T0 was artificially inseminated to recipient cows at 12 h after onset of estrus (not delayed FTAI). Meanwhile, the delayed FTAI was conducted 18-20 h after onset of estrus using the T0, the best of T1 and T2, and the T3 post-thawed semen. Results: The Percoll density gradient centrifugation reduced motility, viability, and intact plasma membrane but increased sperm abnormalities. Meanwhile, the swim-up process increased motility, viability, and intact plasma membrane of sperm cells but decreased sperm abnormalities. Post-thawed semen decreased motility, viability, and intact plasma membrane of sperm cells but increased sperm abnormalities. The sex ratio of the Simmental crossbred offspring was 96.08% and 100% in T1 and T3, respectively, compared to 48.25% and 67.39% in T0 not delayed and delayed FTAI, respectively. Conclusion: The Percoll density gradient centrifugation and swim-up methods are prospective for obtaining male offspring.
KW - Agricultural innovation
KW - Farm productivity
KW - Motility
KW - Pregnancy rate
KW - Sperm morphologic abnormality
KW - Viability
UR - http://www.scopus.com/inward/record.url?scp=85123717396&partnerID=8YFLogxK
U2 - 10.14202/vetworld.2022.102-109
DO - 10.14202/vetworld.2022.102-109
M3 - Article
AN - SCOPUS:85123717396
SN - 0972-8988
VL - 15
SP - 102
EP - 109
JO - Veterinary World
JF - Veterinary World
IS - 1
ER -
