The Endogenous Development of Eimeria tenella in Chickens Injected Subcutaneously with Oocysts Protein as Initially Study of Development of Cecal Coccidiosis Killed Vaccine

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Abstract

The study was carried out to observe the endogenous development of E. tenella histomorphologically in chickens subcutaneously injected with E. tenella oocyst protein. One day old of twenty-four broiler chickens were divided into 2 groups, each group containing twelve chicks. Group 1 was as control group injected subcutaneously on the neck with two doses: the first dose was administered at age 4th day with Freund's Complete Adjuvant (FCA) emulsified in PBS and administered booster dose was injected at 18th day of age with Freund's Incomplete Adjuvant (FICA) emulsified in PBS. Group 2 was injected subcutaneously on the neck with two doses: first dose at 4th day of age with 50 μg E. tenella oocyst protein emulsified in FCA and booster dose was given at 18th day of age with 50 μg oocyst protein emulsified in FICA. After 14 days of booster, the both groups were challenged orally 1 104 of virulent E. tenella. The assessment of endogenous development of E. tenella in chickens evaluated histomorphologically of cecum and oocyst production examination. Injected E. tenella oocyst protein chickens were challenged at 32nd day of age, demonstrated that parasite endogenous development in intestine histomorphologically appeared decreased in proliferation and suppressing oocyst production rate around 68% compared with un-injected chicken. Impaired development of endogenous parasites occurs due to protective immunity resulting from exposure to antigens so that the ability to reproduce and multiply the parasites decreases. The study results demonstrated that relatively sufficient protection against coccidia by use the E. tenella oocyst protein as material of cecal coccidiosis killed vaccine in broiler after challenge. E. tenella oocysts protein can generate protective immunity against homologous challenge through reduction of proliferation parasite and the presence of parasites disabilities.

Original languageEnglish
Article number012037
JournalJournal of Physics: Conference Series
Volume1665
Issue number1
DOIs
Publication statusPublished - 20 Oct 2020
Event2nd International Seminar on Smart Molecule of Natural Resources, ISSMART 2020 - Malang, Indonesia
Duration: 25 Aug 202026 Aug 2020

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