TY - JOUR
T1 - The Endogenous Development of Eimeria tenella in Chickens Injected Subcutaneously with Oocysts Protein as Initially Study of Development of Cecal Coccidiosis Killed Vaccine
AU - Yunus, Muchammad
AU - Suprihati, Endang
AU - Wijaya, Agus
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2020/10/20
Y1 - 2020/10/20
N2 - The study was carried out to observe the endogenous development of E. tenella histomorphologically in chickens subcutaneously injected with E. tenella oocyst protein. One day old of twenty-four broiler chickens were divided into 2 groups, each group containing twelve chicks. Group 1 was as control group injected subcutaneously on the neck with two doses: the first dose was administered at age 4th day with Freund's Complete Adjuvant (FCA) emulsified in PBS and administered booster dose was injected at 18th day of age with Freund's Incomplete Adjuvant (FICA) emulsified in PBS. Group 2 was injected subcutaneously on the neck with two doses: first dose at 4th day of age with 50 μg E. tenella oocyst protein emulsified in FCA and booster dose was given at 18th day of age with 50 μg oocyst protein emulsified in FICA. After 14 days of booster, the both groups were challenged orally 1 104 of virulent E. tenella. The assessment of endogenous development of E. tenella in chickens evaluated histomorphologically of cecum and oocyst production examination. Injected E. tenella oocyst protein chickens were challenged at 32nd day of age, demonstrated that parasite endogenous development in intestine histomorphologically appeared decreased in proliferation and suppressing oocyst production rate around 68% compared with un-injected chicken. Impaired development of endogenous parasites occurs due to protective immunity resulting from exposure to antigens so that the ability to reproduce and multiply the parasites decreases. The study results demonstrated that relatively sufficient protection against coccidia by use the E. tenella oocyst protein as material of cecal coccidiosis killed vaccine in broiler after challenge. E. tenella oocysts protein can generate protective immunity against homologous challenge through reduction of proliferation parasite and the presence of parasites disabilities.
AB - The study was carried out to observe the endogenous development of E. tenella histomorphologically in chickens subcutaneously injected with E. tenella oocyst protein. One day old of twenty-four broiler chickens were divided into 2 groups, each group containing twelve chicks. Group 1 was as control group injected subcutaneously on the neck with two doses: the first dose was administered at age 4th day with Freund's Complete Adjuvant (FCA) emulsified in PBS and administered booster dose was injected at 18th day of age with Freund's Incomplete Adjuvant (FICA) emulsified in PBS. Group 2 was injected subcutaneously on the neck with two doses: first dose at 4th day of age with 50 μg E. tenella oocyst protein emulsified in FCA and booster dose was given at 18th day of age with 50 μg oocyst protein emulsified in FICA. After 14 days of booster, the both groups were challenged orally 1 104 of virulent E. tenella. The assessment of endogenous development of E. tenella in chickens evaluated histomorphologically of cecum and oocyst production examination. Injected E. tenella oocyst protein chickens were challenged at 32nd day of age, demonstrated that parasite endogenous development in intestine histomorphologically appeared decreased in proliferation and suppressing oocyst production rate around 68% compared with un-injected chicken. Impaired development of endogenous parasites occurs due to protective immunity resulting from exposure to antigens so that the ability to reproduce and multiply the parasites decreases. The study results demonstrated that relatively sufficient protection against coccidia by use the E. tenella oocyst protein as material of cecal coccidiosis killed vaccine in broiler after challenge. E. tenella oocysts protein can generate protective immunity against homologous challenge through reduction of proliferation parasite and the presence of parasites disabilities.
UR - http://www.scopus.com/inward/record.url?scp=85096354498&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1665/1/012037
DO - 10.1088/1742-6596/1665/1/012037
M3 - Conference article
AN - SCOPUS:85096354498
SN - 1742-6588
VL - 1665
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012037
T2 - 2nd International Seminar on Smart Molecule of Natural Resources, ISSMART 2020
Y2 - 25 August 2020 through 26 August 2020
ER -
