TY - JOUR
T1 - The efficacy of human dental pulp stem cells in regenerating submandibular gland defects in diabetic wistar rats (Rattus novergicus)
AU - Suciadi, Septiana P.
AU - Nugraha, Alexander P.
AU - Ernawati, Diah S.
AU - Ayuningtyas, Nurina F.
AU - Narmada, Ida B.
AU - Prahasanti, Chiquita
AU - Dinaryanti, Aristika
AU - Ihsan, Igo Syaiful
AU - Hendrinto, Eryk
AU - Susilowati, Helen
AU - Rantam, Fedik Abdul
N1 - Funding Information:
The research was funded by the Penelitian Dasar Unggulan Perguruan Tinggi (PDUPT) of the Ministry of Research, Technology and Higher Education of the Republic of Indonesia (Kemenristekdikti RI) (letter of appointment grant number 893/UN3/ 2018). The authors would like to thank the Faculty of Medicine, Faculty of Dental Medicine, Faculty of Veterinary Medicine, Stem Cell Research and Development Centre, Universitas Airlangga for its support of the research reported here.
Publisher Copyright:
© RJPT All right reserved.
PY - 2019
Y1 - 2019
N2 - Chronic hyperglicemia in Diabetes Mellitus caused microangiopathy in salivary gland. Human Dental Pulp Stem Cells (HDPSCs) suspected can regenerate the defect. The aim of this study was to analyze the efficacy of HDPSCs in stimulating angiogenesis, acinar cell numbers and Transforming Growth Factor-β (TGF-β) serum to regenerate submandibular gland defects in diabetic Wistar rats. Twenty-four male Wistar (250-350 g) rats 3-months-old were used. Rats were divided into 4 groups (n=6 each: a positive control group on Day 7 (DM) (C+7), a positive control group on Day 14 (DM) (C+14), a treatment group on Day 7 (DM+5.105 HDPSCs transplantation intraglandular) (T7) and a treatment group on Day 14 (DM+5.105 HDPSCs transplantation intraglandular) (T14). Wistar Rats were administered with 30 mg of Streptozotocin per kg of bodyweight to induce Diabetes Mellitus (DM). Histopathological examination with HE staining was performed to analyse neovascularization and acinar cell numbers. ELISA was performed to measure TGF-β serum. Statistical analysis used: A Tukey HSD or Bonferroni test after ANOVA or Kruskal Wallis test was performed (p<0.05) based on a Saphiro Wilk and Levene’s test (p>0.05). The highest acinar cell number was found in the T7 group [513.167±136.17] with no significant difference [p=0.136, p<0.05]. The highest capillaries were found in T14 [10.667±4.54] and TGF-β serum level [168.87±37.38] with significant difference [p=0.006; p<0.05] and [p=0.008, p<0.05]. HDPSCs can regenerate submandibular gland defects in Diabetic Wistar rats by stimulating angiogenesis, acinar cells number and TGF – β serum.
AB - Chronic hyperglicemia in Diabetes Mellitus caused microangiopathy in salivary gland. Human Dental Pulp Stem Cells (HDPSCs) suspected can regenerate the defect. The aim of this study was to analyze the efficacy of HDPSCs in stimulating angiogenesis, acinar cell numbers and Transforming Growth Factor-β (TGF-β) serum to regenerate submandibular gland defects in diabetic Wistar rats. Twenty-four male Wistar (250-350 g) rats 3-months-old were used. Rats were divided into 4 groups (n=6 each: a positive control group on Day 7 (DM) (C+7), a positive control group on Day 14 (DM) (C+14), a treatment group on Day 7 (DM+5.105 HDPSCs transplantation intraglandular) (T7) and a treatment group on Day 14 (DM+5.105 HDPSCs transplantation intraglandular) (T14). Wistar Rats were administered with 30 mg of Streptozotocin per kg of bodyweight to induce Diabetes Mellitus (DM). Histopathological examination with HE staining was performed to analyse neovascularization and acinar cell numbers. ELISA was performed to measure TGF-β serum. Statistical analysis used: A Tukey HSD or Bonferroni test after ANOVA or Kruskal Wallis test was performed (p<0.05) based on a Saphiro Wilk and Levene’s test (p>0.05). The highest acinar cell number was found in the T7 group [513.167±136.17] with no significant difference [p=0.136, p<0.05]. The highest capillaries were found in T14 [10.667±4.54] and TGF-β serum level [168.87±37.38] with significant difference [p=0.006; p<0.05] and [p=0.008, p<0.05]. HDPSCs can regenerate submandibular gland defects in Diabetic Wistar rats by stimulating angiogenesis, acinar cells number and TGF – β serum.
KW - Acinar cells
KW - Angiogenesis
KW - Diabetes mellitus
KW - Human dental pulp stem cells
KW - Submandibular gland defect
KW - Transforming growth factor-β
UR - http://www.scopus.com/inward/record.url?scp=85066472072&partnerID=8YFLogxK
U2 - 10.5958/0974-360X.2019.00261.0
DO - 10.5958/0974-360X.2019.00261.0
M3 - Article
AN - SCOPUS:85066472072
SN - 0974-3618
VL - 12
SP - 1573
EP - 1579
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 4
ER -
