Introduction: The production of numerous growth factors is an essential feature of conditioned medium of amniotic membrane stem cells (AMSC-CM). Trans epidermal delivery is a new potential method for rejuvenation in photoaging skin. This study set out to compare the efficacy of fractional CO2 laser (CO2 laser) and microneedling for AMSC-CM in photoaging patients. Methods: An analytical method experiment was conducted on 60 women who were randomly classified into two groups; treated with AMSC-CM either after microneedling and CO2 laser. Both groups were applied AMSC-CM topically for three times. The dynamic of clinical photoaging process was assessed by analysing wrinkle, pore, polarized spot, UV spot, and skin tone. Results: There was a statistically significant difference in delta polarized spot, UV spot, and pore of the third to first evaluation, between the two groups (p< 0.05). Besides, the median difference (delta) of polarized spot, UV spot, and pore parameter in the microneedling group was notably better than in the laser group. The median of skin tone of the fractional CO2 laser plus AMSC-CM demonstrated statistically improvements between first to second evaluation (p=0.04). Conclusion: CO2 laser and microneedling can accelerate efficacy of AMSC-CM trans epidermal delivery for the treatment of photoaging. The microneedling plus AMSC-CM is considered to be more effective for improving the pore, polarized spot, and ultraviolet spot.

Original languageEnglish
Pages (from-to)91-97
Number of pages7
JournalMalaysian Journal of Medicine and Health Sciences
Publication statusPublished - Sept 2021


  • Fractional CO laser
  • Microneedling
  • Photoaging
  • Trans epidermal delivery


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