TY - JOUR
T1 - The effects of insulin-like growth factor I (IGF-I) complex from seminal plasma on capacitation, membrane integrity and DNA fragmentation in goat spermatozoa
AU - Susilowati, Suherni
AU - Triana, Indah Norma
AU - Malik, Abdul
N1 - Publisher Copyright:
© 2015 Hainan Medical College.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - To evaluate the effects of the insulin-like growth factor I (IGF-I) complex from seminal plasma on capacitation, membrane integrity and DNA fragmentation. Methods: A total of 0.5mL of fresh semen was added to 1mL of Bracket-Oliphant (BO) medium, and the sample was then centrifuged at a speed of 1800rpm for 10min. The samples were analyzed before and after centrifugation for sperm viability, motility, membrane integrity and capacitation. The centrifuged samples were divided into three groups, each consisting of 3 × 106 spermatozoa. BO medium was added to group 1, BO+12ng IGF-I complex medium was added to group 2, and 12ng IGF-I complex was added to group 3. Then, the samples were incubated for 15min. Results: The result showed that sperm motility, viability and membrane integrity were significantly higher (P<0.05) after centrifugation. Furthermore, the sperm capacitation was significantly lower (P<0.05) after centrifugation. The percentages of sperm capacitation, membrane integrity and DNA fragmentation were significantly different (P<0.05) in all media, including BO, BO+IGF-I complex and the IGF-I complex alone. Conclusions: Sperm quality include motility, viability and membrane integrity were lower after centrifugation. Whereas DNA fragmentation after incubation in the IGF-I complex medium also was lower compared to that of specimens in the BO and BO+IGF-I complex media.
AB - To evaluate the effects of the insulin-like growth factor I (IGF-I) complex from seminal plasma on capacitation, membrane integrity and DNA fragmentation. Methods: A total of 0.5mL of fresh semen was added to 1mL of Bracket-Oliphant (BO) medium, and the sample was then centrifuged at a speed of 1800rpm for 10min. The samples were analyzed before and after centrifugation for sperm viability, motility, membrane integrity and capacitation. The centrifuged samples were divided into three groups, each consisting of 3 × 106 spermatozoa. BO medium was added to group 1, BO+12ng IGF-I complex medium was added to group 2, and 12ng IGF-I complex was added to group 3. Then, the samples were incubated for 15min. Results: The result showed that sperm motility, viability and membrane integrity were significantly higher (P<0.05) after centrifugation. Furthermore, the sperm capacitation was significantly lower (P<0.05) after centrifugation. The percentages of sperm capacitation, membrane integrity and DNA fragmentation were significantly different (P<0.05) in all media, including BO, BO+IGF-I complex and the IGF-I complex alone. Conclusions: Sperm quality include motility, viability and membrane integrity were lower after centrifugation. Whereas DNA fragmentation after incubation in the IGF-I complex medium also was lower compared to that of specimens in the BO and BO+IGF-I complex media.
KW - DNA fragmentation
KW - IGF-I complex
KW - Membraneintegrity
KW - Seminal plasma
KW - Spermatozoa
UR - http://www.scopus.com/inward/record.url?scp=85006121577&partnerID=8YFLogxK
U2 - 10.1016/j.apjr.2015.06.003
DO - 10.1016/j.apjr.2015.06.003
M3 - Article
AN - SCOPUS:85006121577
SN - 2305-0500
VL - 4
SP - 208
EP - 211
JO - Asian Pacific Journal of Reproduction
JF - Asian Pacific Journal of Reproduction
IS - 3
ER -